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Pathogenic characterization and mechanism of sequence type 4 Cronobacter sakazakii derived from milk-based infant and baby foods
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Pathogenic characterization and mechanism of sequence type 4 Cronobacter sakazakii derived from milk-based infant and baby foods
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Journal Article
Pathogenic characterization and mechanism of sequence type 4 Cronobacter sakazakii derived from milk-based infant and baby foods
2025
Overview
Background
Cronobacter sakazakii
can cause severe infections in premature infants and neonates through the consumption of contaminated milk-based foods. However, the pathogenesis of sequence type 4 (ST4)
C. sakazakii
remains to be fully elucidated.
Results
In this study, four ST4
C. sakazakii
strains were investigated via comparative toxicity, genomic, and transcriptomic analyses to elucidate their pathogenic characteristics and mechanisms. Multilocus sequence typing (MLST) indicated that ST4
C. sakazakii
was frequently identified among 36
Cronobacter
spp. isolates recovered from milk-based infant and baby foods, and 13 novel STs were also detected. Compared with other ST isolates, ST4
C. sakazakii
displayed a higher gut weight to carcass weight ratio (GW/CW), stronger abilities to invade and translocate, and increased secretion of TNF-α, IL-1, and lactate dehydrogenase (LDH) in human brain microvascular endothelial cells (HBMECs) and human U251 glioma cells (U251). Moreover, ST4
C. sakazakii
strains with a higher GW/CW ratio significantly disrupted routine blood indices, promoted the secretion of inflammatory factors, and induced severe histopathological changes in the liver, brain, spleen, kidney, and intestine of suckling mice. Although differences in genome composition and known virulence factors were observed among these ST4
C. sakazakii
strains with varying pathogenic phenotypes, comparative transcriptomic analyses revealed that the expression of numerous virulence factors and pathways, including
ompA, ompW, luxS, rpoS
, the Sec secretion system, lipopolysaccharide biosynthesis and assembly, and flagellar assembly, greatly contributed to the high pathogenicity of ST4
C. sakazakii
.
Conclusion
Our findings suggest that foodborne ST4
C. sakazakii
isolates represent a significant potential threat to food safety and public health, particularly for premature and immunocompromised infants.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Animals
/ Babies
/ Biomedical and Life Sciences
/ Brain
/ Comparative toxicity analysis
/ Comparative transcriptome analysis
/ Cronobacter sakazakii - classification
/ Cronobacter sakazakii - genetics
/ Cronobacter sakazakii - isolation & purification
/ Cronobacter sakazakii - pathogenicity
/ Enterobacteriaceae Infections - microbiology
/ Enterobacteriaceae Infections - pathology
/ Flagella
/ Food
/ Humans
/ Infant
/ Infants
/ Mice
/ Milk
/ Mycology
/ Neonates
/ Prevalence studies (Epidemiology)
/ Toxicity
/ Toxins
/ Virology
