Journal Article

Transcriptome analysis reveals gene responses to herbicide, tribenuron methyl, in Brassica napus L. during seed germination

Lei, Wei, Shi, Hongsong, Yuan, Fang, Zhou, Qingyuan, Wang, Ruili, Meng, Lijiao, Wu, Jiayi, Cui, Cui, Wang, Liuyan, Li, Chenyang

2021

Overview

Background Tribenuron methyl (TBM) is an herbicide that inhibits sulfonylurea acetolactate synthase (ALS) and is one of the most widely used broad-leaved herbicides for crop production. However, soil residues or drifting of the herbicide spray might affect the germination and growth of rapeseed, Brassica napus , so it is imperative to understand the response mechanism of rape to TBM during germination. The aim of this study was to use transcriptome analysis to reveal the gene responses in herbicide-tolerant rapeseed to TBM stress during seed germination. Results 2414, 2286, and 1068 differentially expressed genes (DEGs) were identified in TBM-treated resistant vs sensitive lines, treated vs. control sensitive lines, treated vs. control resistant lines, respectively. GO analysis showed that most DEGs were annotated to the oxidation-reduction pathways and catalytic activity. KEGG enrichment was mainly involved in plant-pathogen interactions, α-linolenic acid metabolism, glucosinolate biosynthesis, and phenylpropanoid biosynthesis. Based on GO and KEGG enrichment, a total of 137 target genes were identified, including genes involved in biotransferase activity, response to antioxidant stress and lipid metabolism. Biotransferase genes, CYP450, ABC and GST , detoxify herbicide molecules through physical or biochemical processes. Antioxidant genes, RBOH, WRKY, CDPK, MAPK, CAT, and POD regulate plant tolerance by transmitting ROS signals and triggering antioxidant enzyme expression. Lipid-related genes and hormone-related genes were also found, such as LOX3, ADH1, JAZ6, BIN2 and ERF , and they also played an important role in herbicide resistance. Conclusions This study provides insights for selecting TBM-tolerant rapeseed germplasm and exploring the molecular mechanism of TBM tolerance during germination.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Acetolactate synthase / Acids / Animal Genetics and Genomics / Antioxidants / Arylsulfonates / Biomedical and Life Sciences / Biosynthesis / Brassica / Brassica napus / Brassica napus - genetics / Brassica napus L / Catalytic activity / Crop production / Crop residues / Gene expression / Gene Expression Profiling / Genes / Genetic aspects / Genomes / Genomics / Germination / Germination - genetics / Germplasm / Grain / Growth / Herbicide resistance / Herbicides / Herbicides - toxicity / Kinases / Life Sciences / Linolenic acid / Lipid metabolism / Lipids / MAP kinase / Metabolism / Metabolites / Microarrays / Microbial Genetics and Genomics / Oxidation / Oxidation-reduction potential / Pathogens / Physiology / Plant Genetics and Genomics / Plant genomics / Proteins / Proteomics / Rape plants / Rapeseed / Research Article / Seed germination / Seeds / Signal transduction / Sulfonylurea / Target recognition / Transcriptome / Transcriptomes / Tribenuron methyl