Journal Article

The First Virome of a Russian Vineyard

Massart, Sébastien, Varallyay, Eva, Vinogradova, Svetlana, Navrotskaya, Emiliya, Porotikova, Elena, Galbacs, Zsuzsanna Nagyne

2023

Overview

Among other pathogens, more than 80 viruses infect grapevine. The aim of this work was to study the virome diversity of grapevine viruses and mycoviruses of a vineyard using high-throughput sequencing technologies. The grapevine virome was studied in symptomatic vines of the Rkatsiteli cultivar (V. vinifera) collected at the vineyards of the Krasnodar Krai in Russia. Ribosomal-depleted total RNA and isolated small RNAs were used for library preparation and high-throughput sequencing. Six grapevine-infecting viruses and two viroids were validated by RT-PCR and analyzed phylogenetically. We identified the presence of grapevine leafroll-associated virus 3, grapevine Pinot gris virus, grapevine virus T, grapevine rupestris stem-pitting-associated virus, grapevine fleck virus, and grapevine rupestris vein feathering virus, as well as two viroids, grapevine yellow speckle viroid 1 and hop stunt viroid. We also studied the mycovirome of the vineyard and identified nine viruses with single-stranded positive-sense RNA genomes: alternaria arborescens mitovirus 1, botrytis cinerea mitovirus 1, botrytis cinerea mitovirus 2, botrytis cinerea mitovirus 3, botrytis cinerea mitovirus 4, sclerotinia sclerotiorum mitovirus 3, botrytis cinerea hypovirus 1, grapevine-associated narnavirus 1, and botrytis virus F. In addition, we identified botrytis cinerea hypovirus 1 satellite-like RNA and two single-stranded negative-sense RNA viruses. This is the first study of grapevine mycoviruses in Russia. The obtained result will contribute to the development of biocontrol strategies in the future.

• Publisher: MDPI AG,MDPI
• Subject: Alternaria arborescens / Ambiguity / Analysis / Biological control / Biotechnologie / Biotechnology / Botrytis cinerea / Botrytis virus F / Cultivars / Diseases and pests / Ecology / Ecology, Evolution, Behavior and Systematics / Environmental aspects / Fungi / genome / Genomes / Genomics / Grapes / Grapevine fleck virus / Grapevine leafroll-associated virus 3 / grapevine viruses / Grapevine yellow speckle viroid 1 / Grapevines / high-throughput sequencing / Hop stunt viroid / Life sciences / Microorganisms / mycoviruses / Next-generation sequencing / Pathogens / Pests / Phylogenetics / Phylogeny / Physiological aspects / Plant Science / Plant viruses / RNA / RNA viruses / rRNA / Russia / Satellite RNA / Sciences du vivant / Sclerotinia sclerotiorum / Vineyards / Viral infections / viral metagenomics / Viroids / virome / Virus diseases of plants / Viruses / Vitis / Vitis vinifera / Wine / Wine industry / Wineries / Wineries & vineyards