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Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells
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Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells
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Journal Article
Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells
2014
Overview
A seven-stage protocol enables differentiation of human embryonic stem cells into cells similar to pancreatic beta cells.
Transplantation of pancreatic progenitors or insulin-secreting cells derived from human embryonic stem cells (hESCs) has been proposed as a therapy for diabetes. We describe a seven-stage protocol that efficiently converts hESCs into insulin-producing cells. Stage (S) 7 cells expressed key markers of mature pancreatic beta cells, including MAFA, and displayed glucose-stimulated insulin secretion similar to that of human islets during static incubations
in vitro
. Additional characterization using single-cell imaging and dynamic glucose stimulation assays revealed similarities but also notable differences between S7 insulin-secreting cells and primary human beta cells. Nevertheless, S7 cells rapidly reversed diabetes in mice within 40 days, roughly four times faster than pancreatic progenitors. Therefore, although S7 cells are not fully equivalent to mature beta cells, their capacity for glucose-responsive insulin secretion and rapid reversal of diabetes
in vivo
makes them a promising alternative to pancreatic progenitor cells or cadaveric islets for the treatment of diabetes.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
