Journal Article

High salt diet impairs dermal tissue remodeling in a mouse model of IMQ induced dermatitis

Veréb, Zoltán, Pap, Domonkos, Szebeni, Beáta, Dobosy, Péter, Kemény, Lajos, Tulassay, Tivadar, Pajtók, Csenge, Vannay, Ádám, Agócs, Róbert, Szabó, Attila J., Németh, István, Veres-Székely, Apor

2021

Overview

Recent animal studies, as well as quantitative sodium MRI observations on humans demonstrated that remarkable amounts of sodium can be stored in the skin. It is also known that excess sodium in the tissues leads to inflammation in various organs, but its role in dermal pathophysiology has not been elucidated. Therefore, our aim was to study the effect of dietary salt loading on inflammatory process and related extracellular matrix (ECM) remodeling in the skin. To investigate the effect of high salt consumption on inflammation and ECM production in the skin mice were kept on normal (NSD) or high salt (HSD) diet and then dermatitis was induced with imiquimod (IMQ) treatment. The effect of high salt concentration on dermal fibroblasts (DF) and peripheral blood mononuclear cells (PBMC) was also investigated in vitro . The HSD resulted in increased sodium content in the skin of mice. Inflammatory cytokine Il17 expression was elevated in the skin of HSD mice. Expression of anti-inflammatory Il10 and Il13 decreased in the skin of HSD or HSD IMQ mice. The fibroblast marker Acta2 and ECM component Fn and Col1a1 decreased in HSD IMQ mice. Expression of ECM remodeling related Pdgfb and activation phosphorylated (p)-SMAD2/3 was lower in HSD IMQ mice. In PBMCs, production of IL10 , IL13 and PDGFB was reduced due to high salt loading. In cultured DFs high salt concentration resulted in decreased cell motility and ECM production, as well. Our results demonstrate that high dietary salt intake is associated with increased dermal pro-inflammatory status. Interestingly, although inflammation induces the synthesis of ECM in most organs, the expression of ECM decreased in the inflamed skin of mice on high salt diet. Our data suggest that salt intake may alter the process of skin remodeling.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Analysis / Animal tissues / Animals / Biology and Life Sciences / Biomarkers - metabolism / Body Weight / Cell Movement / Cells, Cultured / Collagen (type I) / Committees / Complications and side effects / Computer and Information Sciences / Cytokines / Cytokines - metabolism / Dermatitis / Dermatitis - pathology / Dermatology / Dermis - pathology / Diet / Dietary intake / Disease Models, Animal / Engineering and Technology / Experiments / Extracellular matrix / Extracellular Matrix - metabolism / Fibroblasts / Fibroblasts - pathology / Health aspects / Homeostasis / Humans / Imiquimod / Imiquimod - adverse effects / Inflammation / Inflammation - pathology / Inflammation Mediators - metabolism / Interleukin 1 / Interleukin 10 / Interleukin 13 / Interleukin 17 / Laboratory animals / Leukocytes (mononuclear) / Leukocytes, Mononuclear - metabolism / Magnetic resonance imaging / Male / Medical research / Medicine and Health Sciences / Mice / Mice, Inbred C57BL / Nephrology / Organs / Patient outcomes / Pediatrics / Penicillin / Peripheral blood mononuclear cells / Physical Sciences / Probiotics - metabolism / Research and Analysis Methods / Risk factors / Salt / Salt loading / Salts / Skin / Smad2 protein / Sodium / Sodium Chloride, Dietary - adverse effects / Sodium content