Asset Details
Do you wish to reserve the book?
Reprogramming the unfolded protein response for replication by porcine reproductive and respiratory syndrome virus
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Reprogramming the unfolded protein response for replication by porcine reproductive and respiratory syndrome virus
Do you wish to request the book?
Reprogramming the unfolded protein response for replication by porcine reproductive and respiratory syndrome virus
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Reprogramming the unfolded protein response for replication by porcine reproductive and respiratory syndrome virus
2019
Overview
The unfolded protein response (UPR) in the endoplasmic reticulum (ER) constitutes a critical component of host innate immunity against microbial infections. In this report, we show that porcine reproductive and respiratory syndrome virus (PRRSV) utilizes the UPR machinery for its own benefit. We provide evidence that the virus targets the UPR central regulator GRP78 for proteasomal degradation via a mechanism that requires viral glycoprotein GP2a, while both IRE1-XBP1s and PERK-eIF2α-ATF4 signaling branches of the UPR are turned on at early stage of infection. The activated effector XBP1s was found to enter the nucleus, but ATF4 was unexpectedly diverted to cytoplasmic viral replication complexes by means of nonstructural proteins nsp2/3 to promote viral RNA synthesis. RNAi knockdown of either ATF4 or XBP1s dramatically attenuated virus titers, while overexpression caused increases. These observations reveal attractive host targets (e.g., ATF4 and XBP1s) for antiviral drugs and have implications in vaccine development.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
Activating Transcription Factor 4 - genetics
/ Activating Transcription Factor 4 - metabolism
/ Animals
/ Endoplasmic Reticulum - metabolism
/ Endoplasmic Reticulum - virology
/ Heat-Shock Proteins - genetics
/ Heat-Shock Proteins - metabolism
/ Kinases
/ Medicine and Health Sciences
/ Porcine reproductive and respiratory syndrome
/ Porcine Reproductive and Respiratory Syndrome - genetics
/ Porcine Reproductive and Respiratory Syndrome - metabolism
/ Porcine Reproductive and Respiratory Syndrome - virology
/ Porcine respiratory and reproductive syndrome virus - physiology
/ Proteins
/ Research and analysis methods
/ RNA
/ Sensors
/ Swine
/ Vaccines
/ Viruses
