Asset Details
Do you wish to reserve the book?
The protective effect of carbamazepine on acute lung injury induced by hemorrhagic shock and resuscitation in rats
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
The protective effect of carbamazepine on acute lung injury induced by hemorrhagic shock and resuscitation in rats
Do you wish to request the book?
The protective effect of carbamazepine on acute lung injury induced by hemorrhagic shock and resuscitation in rats
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
The protective effect of carbamazepine on acute lung injury induced by hemorrhagic shock and resuscitation in rats
2024
Overview
Hemorrhagic shock and resuscitation (HSR) enhances the risk of acute lung injury (ALI). This study investigated the protective effect of carbamazepine (CBZ) on HSR-induced ALI in rats. Male Sprague-Dawley rats were allocated into five distinct groups through randomization: control (SHAM), saline + HSR (HSR), CBZ + HSR (CBZ/HSR), dimethyl sulfoxide (DMSO) + HSR (DMSO/HSR), and CBZ + chloroquine (CQ) + HSR (CBZ/CQ/HSR). Subsequently, HSR models were established. To detect tissue damage, we measured lung histological changes, lung injury scores, and wet/dry weight ratios. We measured neutrophil counts as well as assessed the expression of inflammatory factors using RT-PCR to determine the inflammatory response. We detected autophagy-related proteins LC3II/LC3I, P62, Beclin-1, and Atg12-Atg5 using western blotting. Pretreatment with CBZ improved histopathological changes in the lungs and reduced lung injury scores. The CBZ pretreatment group exhibited significantly reduced lung wet/dry weight ratio, neutrophil aggregation and number, and inflammation factor ( TNF-α and iNOS ) expression. CBZ changed the expression levels of autophagy-related proteins (LC3II/LC3I, beclin-1, Atg12-Atg5, and P62), suggesting autophagy activation. However, after injecting CQ, an autophagy inhibitor, the beneficial effects of CBZ were reversed. Taken together, CBZ pretreatment improved HSR-induced ALI by suppressing inflammation, at least in part, through activating autophagy. Thus, our study offers a novel perspective for treating HSR-induced ALI.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Acute Lung Injury - diagnosis
/ Acute Lung Injury - immunology
/ Acute Lung Injury - prevention & control
/ Acute respiratory distress syndrome
/ Animals
/ Carbamazepine - administration & dosage
/ Complications and side effects
/ Inflammation Mediators - analysis
/ Inflammation Mediators - metabolism
/ Male
/ Medicine and Health Sciences
/ Protective Agents - administration & dosage
/ Proteins
/ Rats
/ Resuscitation - adverse effects
/ Shock, Hemorrhagic - complications
/ Shock, Hemorrhagic - immunology
/ Shock, Hemorrhagic - therapy
/ Testing
