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Trichoderma lixii (IIIM-B4), an endophyte of Bacopa monnieri L. producing peptaibols
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Trichoderma lixii (IIIM-B4), an endophyte of Bacopa monnieri L. producing peptaibols
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Journal Article
Trichoderma lixii (IIIM-B4), an endophyte of Bacopa monnieri L. producing peptaibols
2019
Overview
Background
Exploration of microbes isolated from north western Himalayas for bioactive natural products.
Results
A strain of
Trichoderma lixii
(IIIM-B4) was isolated from
Bacopa monnieri
L. The ITS based rDNA gene sequence of strain IIIM-B4 displayed 99% sequence similarity with different
Trichoderma harzianum
species complex. The highest score was displayed for
Hypocrea lixii
strain FJ462763 followed by
H. nigricans
strain NBRC31285,
Trichoderma lixii
strain CBS 110080,
T. afroharzianum
strain CBS124620 and
Trichoderma guizhouense
BPI:GJS 08135 respectively. Position of
T. lixii
(IIIM-B4) in phylogenetic tree suggested separate identity of the strain. Microbial dynamics of
T. lixii
(IIIM-B4) was investigated for small peptides. Medium to long chain length peptaibols of 11 residue (Group A), 14 residue (Group B) and 17 residue (Group C) were identified using Matrix Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometer. Optimization is undeniably a desideratum for maximized production of desirable metabolites from microbial strain. Here optimization studies were carried out on
T. lixii
(IIIM-B4) using different growth media through Intact Cell Mass Spectrometry (ICMS). A multifold increase was obtained in production of 11 residue peptaibols using rose bengal medium. Out of these, one of them named as Tribacopin AV was isolated and sequenced through mass studied. It was found novel as having unique sequence Ac-Gly-Leu-Leu-Leu-Ala-Leu-Pro-Leu-Aib-Val-Gln-OH. It was found to have antifungal activity against
Candida albicans
(25 μg/mL MIC).
Conclusion
In this study, we isolated a strain of
T. lixii
(IIIM-B4) producing medium and long chain peptaibols. One of them named as Tribacopin AV was found novel as having unique sequence Ac-Gly-Leu-Leu-Leu-Ala-Leu-Pro-Leu-Aib-Val-Gln-OH, which had antifungal properties.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Antifungal Agents - isolation & purification
/ Antifungal Agents - pharmacology
/ Bacopa
/ Biological Products - isolation & purification
/ Biological Products - pharmacology
/ Biomedical and Life Sciences
/ Candida albicans - drug effects
/ Chains
/ Chromatography, High Pressure Liquid
/ DNA
/ Fungi
/ Genes
/ Glycine
/ Intact cell mass spectrometry
/ Matrix assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometer
/ Minimum inhibitory concentration
/ Mycology
/ Novels
/ Peptides
/ Residues
/ Virology
