Journal Article

A standardised framework to identify optimal animal models for efficacy assessment in drug development

Moors, Ellen H. M., Gispen-de Wied, Christine C., S. Ferreira, Guilherme, Veening-Griffioen, Désirée H., Schellekens, Huub, van Meer, Peter J. K., Boon, Wouter P. C.

2019

Overview

Poor translation of efficacy data derived from animal models can lead to clinical trials unlikely to benefit patients-or even put them at risk-and is a potential contributor to costly and unnecessary attrition in drug development. To develop a tool to assess, validate and compare the clinical translatability of animal models used for the preliminary assessment of efficacy. We performed a scoping review to identify the key aspects used to validate animal models. Eight domains (Epidemiology, Symptomatology and Natural History-SNH, Genetic, Biochemistry, Aetiology, Histology, Pharmacology and Endpoints) were identified. We drafted questions to evaluate the different facets of human disease simulation. We designed the Framework to Identify Models of Disease (FIMD) to include standardised instructions, a weighting and scoring system to compare models as well as factors to help interpret model similarity and evidence uncertainty. We also added a reporting quality and risk of bias assessment of drug intervention studies in the Pharmacological Validation domain. A web-based survey was conducted with experts from different stakeholders to gather input on the framework. We conducted a pilot study of the validation in two models for Type 2 Diabetes (T2D)-the ZDF rat and db/db mouse. Finally, we present a full validation and comparison of two animal models for Duchenne Muscular Dystrophy (DMD): the mdx mouse and GRMD dog. We show that there are significant differences between the mdx mouse and the GRMD dog, the latter mimicking the human epidemiological, SNH, and histological aspects to a greater extent than the mouse despite the overall lack of published data. FIMD facilitates drug development by serving as the basis to select the most relevant model that can provide meaningful data and is more likely to generate translatable results to progress drug candidates to the clinic.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Analysis / Animal experimentation / Animals / Biology and Life Sciences / Clinical trials / Computer and Information Sciences / Diabetes Mellitus, Type 2 - pathology / Disease Models, Animal / Dogs / Drug Development - methods / Drug discovery / Drugs / Medicine and Health Sciences / Methods / Mice / Models, Animal / Muscular Dystrophy, Duchenne - pathology / Pilot Projects / Rats / Reference Standards / Research and Analysis Methods / Safety and security measures