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Extracellular vesicles in low volume uterine lavage and serum: novel and promising biomarker for endometritis in Arabian mares

by Derbala, Mohamed K. , Sosa, A. S. , Hasanain, M. H. , Saber, Yasser H. A. , Mahmoud, Karima Gh. M. , Seidel, George E. , Ahmed, Youssef , Ibrahim, Sally , Nawito, M. F. , Taqi, Mohamed O. , Hedia, Mohamed

in Animals / Bacteria / Biomarkers / blood serum / CD63 antigen / CD9 antigen / cell communication / Cell interactions / Cell membranes / Cytokines / Diagnosis / Dinoprostone / Electron microscopy / Endometritis / Endometritis - diagnosis / Endometritis - veterinary / Extracellular Vesicles / Female / Fertility / Flow cytometry / Genetic aspects / Health aspects / Horse Diseases - diagnosis / Horses / Infertility / Inflammation / Interleukin 6 / Light scattering / Mares / Medicine / Medicine & Public Health / Microscopy / Morphology / Ovaries / Physiology / Pregnancy / Prostaglandin E2 / Prostaglandins / Serum / Serum levels / Sperm / Therapeutic Irrigation - veterinary / Transgenics / Ultracentrifugation / Uterine lavage / Uterus / Veterinary Medicine/Veterinary Science / Zoology

2022

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Extracellular vesicles in low volume uterine lavage and serum: novel and promising biomarker for endometritis in Arabian mares

2022

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Extracellular vesicles in low volume uterine lavage and serum: novel and promising biomarker for endometritis in Arabian mares

2022

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Journal Article

Extracellular vesicles in low volume uterine lavage and serum: novel and promising biomarker for endometritis in Arabian mares

Derbala, Mohamed K.,

Sosa, A. S.,

Hasanain, M. H.,

Saber, Yasser H. A.,

Mahmoud, Karima Gh. M.,

Seidel, George E.,

Ahmed, Youssef,

Ibrahim, Sally,

Nawito, M. F.,

Taqi, Mohamed O.,

Hedia, Mohamed

2022

Overview

Background Extracellular vesicles (EVs) are a promising biomarker and play a vital role in cell–cell communication. This study aimed (I) to identify and characterize EVs from low volume uterine lavage (LVL) and serum in mares with endometritis, compared to healthy controls and (II) to measure serum levels of interleukin 6 (IL-6), and prostaglandins (PGF 2α and PGE 2 ). Mares were divided into 30 sub-fertile (endometritis) and 20 fertile (controls). Serum and LVL was collected for EV isolation, and determination of serum levels of inflammatory mediators. Characterization and visualization of EVs were done by electron microscopy, dynamic light scattering and flow cytometry. Results Serial ultracentrifugation of LVL and use of a commercial kit for serum were strategies for EVs isolation. Mares with endometritis released higher amounts of larger size EVs. The EVs from mares with endometritis differentially expressed CD9 and CD63, compared to controls. Mares suffering from endometritis evoked higher levels of inflammatory mediators. Conclusions Thus, EVs could be used for a better understanding the regulatory mechanisms associated with developing endometritis in mares.

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BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC

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