Journal Article

Pluchea dioscoridis extract: A novel therapeutic approach for polycystic ovary syndrome targeting ovarian morphology, dopamine pathways, and neurobehavior in relation to its phytocomponents

Alqurashi, Thamer, Atta, Rasha, Elshamy, Abdelsamed I., Zayed, Mohamed A., Farag, Mohamed A., Gouda, Sahar Galal, Yousof, Shimaa Mohammad, Afifi, Sherif M., Mohamed, Marwa Hussein, Nassar, Mahmoud I.

2025

Overview

Polycystic ovarian syndrome (PCOS) is a prevalent endocrine condition associated with hormonal and metabolic abnormalities, as well as behavioral modifications. This research assesses the impact of the chemically profiled Pluchea dioscoridis ethanolic extract and metformin in a letrozole-induced polycystic ovary syndrome rat model. Thirty female Sprague Dawley rats were allocated into five groups: Control, P. dioscoridis (100 mg/kg), PCOS (letrozole, 1 mg/kg), PCOS + Metformin (300 mg/kg), and PCOS + P. dioscoridis (100 mg/kg), letrazole was given for 8 weeks followed by metformin or P. dioscoridis for 21 days. Behavioral assessments, hormone analyses, and dopamine quantifications in the brain were performed. Ovarian histology and immunohistochemical analysis were conducted. Letrozole-induced PCOS resulted in heightened depression- and anxiety-like behaviors, along with significant hormonal imbalances compared to the control group (P < 0.05%). Both P. dioscoridis and metformin therapies significantly ameliorated these changes, with P. dioscoridis demonstrating better efficacy. P. dioscoridis medication regulated luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone, and estrogen levels, concurrently enhancing cerebral dopamine levels. Histological analysis revealed less cystic follicles and a reinstated normal ovarian architecture in rats treated with P. dioscoridis compared to PCOS group (P < 0.05%). Several flavonoids, nitrogenous compounds and hydroxy cinnamic acid esters were detected in P. dioscoridis samples for the first time utilizing UPLC-ESI-MS analysis. The P. dioscoridis ethanolic extract exhibited potential medicinal properties that are equivalent to or surpass those of metformin for treating behavioral, hormonal, and ovarian structural abnormalities produced by PCOS. It significantly improved dopamine levels, hormonal balance, and ovarian histology, rendering it a suitable alternative or complementary treatment for PCOS.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Abnormalities / Acne / Androgens / Animals / Anxiety / Behavior, Animal - drug effects / Biology and Life Sciences / Care and treatment / Cinnamic acid / Compositae / Disease Models, Animal / Dopamine / Dopamine - metabolism / Esters / Estrogens / Evaluation / Experiments / Female / Flavonoids / Follicle-stimulating hormone / Follicles / Health aspects / Histology / Infertility / Insulin resistance / Letrozole / Luteinizing hormone / Luteinizing Hormone - blood / Materia medica, Vegetable / Medicine and Health Sciences / Menstruation / Metabolites / Metformin / Metformin - pharmacology / Metformin - therapeutic use / Morphology / Nitriles / Ovaries / Ovary - drug effects / Ovary - metabolism / Ovary - pathology / Physical Sciences / Physiological aspects / Phytochemicals - pharmacology / Plant extracts / Plant Extracts - chemistry / Plant Extracts - pharmacology / Plant Extracts - therapeutic use / Pluchea / Polycystic ovary syndrome / Polycystic Ovary Syndrome - chemically induced / Polycystic Ovary Syndrome - drug therapy / Polycystic Ovary Syndrome - metabolism / Polycystic Ovary Syndrome - pathology / Rats / Rats, Sprague-Dawley / Rodents / Stein-Leventhal syndrome / Swimming / Testosterone / Vagina