Journal Article

Multilocus genotype analysis outlines distinct histories for Trichinella britovi in the neighboring Mediterranean islands of Corsica and Sardinia

La Rosa, Giuseppe, Laboratoire de Recherches sur le Développement de l'Elevage (LRDE) ; Institut National de la Recherche Agronomique (INRA), Pozio, Edoardo, Casabianca, François, Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Istituto Superiore di Sanità = National Institute of Health (ISS), Intituto Zooprofilattico Sperimentale della Sicilia ; Partenaires INRAE, Marucci, Gianluca, DG SANTE of the European Commission, Boireau, Pascal, Bandino, Ennio, Vallee, Isabelle, Organisation Mondiale de la Santé Animale / World Organisation Animal Health [Paris] (OIE), Galati, Fabio, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR) ; École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du trava

2018

Overview

Background: The zoonotic nematode Trichinella britovi was discovered in two neighboring Mediterranean islands of Corsica and Sardinia, almost simultaneously at the beginning of the 21st century. An epidemiological link between the two parasite populations was generally assumed. In 2015, an outbreak of trichinellosis in Nice, the South of France, was reportedly caused by the consumption of raw pork delicatessen imported from Corsica. The aims of the present study were to investigate, by multilocus genotype (MLG) analyses, the hypothesis of the common origin of the Corsican and Sardinian T. britovi foci and to trace \"from fork to farm\" the origin of the pork product, which caused a trichinellosis outbreak in mainland France in 2015. Methods: Sixty-three T. britovi isolates were collected from animals and pork products of Sardinia and Corsica islands and from mainland of Italy, France and Spain. We analyzed genetic variability at four polymorphic microsatellite loci by two independent algorithms, the Bayesian and multivariate analyses, to evaluate the genetic relationships of 1367 single larvae. Results: Trichinella britovi isolates of the two islands showed different genetic structures and the Bayesian analysis revealed a different membership of the two insular populations. Furthermore, two geographically separate genetic groups were identified among Corsican isolates. Lastly, the origin of the pork delicatessen marketed in Nice was linked to a breeder-butcher in Corsica. Conclusions: The low level of genetic admixture of the insular T. britovi isolates suggests that this pathogen colonized the two islands by separate events. On the other hand in Corsica, although the isolates share the same genetic structure, geographically separate isolates showed different membership. We suggest the MLG analysis as a suitable method in supporting epidemiological investigations to trace \"from fork to farm\" insular populations of T. britovi.

• Publisher: CCSD,BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Admixtures / Animal behavior / Animals / Animals, Wild - parasitology / Bayesian analysis / Bayesian theory / Biomedical and Life Sciences / Biomedicine / Corsica / Entomology / Epidemiology / Farms / Fox / Foxes / France / Genetic analysis / Genetic aspects / Genetic structure / Genetic variability / genetic variation / Genotype / Genotypes / Health aspects / Hogs / Humanities and Social Sciences / Hypotheses / Infectious Diseases / Islands / Italy / Larvae / Life Sciences / Meat - parasitology / Meat Products - parasitology / Mediterranean Islands / microsatellite repeats / Microsatellites / Microsatellites (Genetics) / Molecular epidemiology / Multilocus Sequence Typing / Nematoda / Nematodes / Outbreaks / Parasites / Parasitology / pathogens / Pig / Population density / Populations / Pork / Probability theory / Sardinia / Software / Spain / Swine / Swine Diseases - parasitology / Trichinella / Trichinella - classification / Trichinella - genetics / Trichinella - isolation & purification / Trichinella britovi / Trichinellosis / Trichinellosis - parasitology / Trichinellosis - veterinary / trichinosis / Tropical Medicine / Veterinary Medicine/Veterinary Science / Virology / Wild boar / Zoonoses
• ISBN: 0004359131000