Asset Details
Do you wish to reserve the book?
Independence of chromatin conformation and gene regulation during Drosophila dorsoventral patterning
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Independence of chromatin conformation and gene regulation during Drosophila dorsoventral patterning
Do you wish to request the book?
Independence of chromatin conformation and gene regulation during Drosophila dorsoventral patterning
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Independence of chromatin conformation and gene regulation during Drosophila dorsoventral patterning
2021
Overview
The relationship between chromatin organization and gene regulation remains unclear. While disruption of chromatin domains and domain boundaries can lead to misexpression of developmental genes, acute depletion of regulators of genome organization has a relatively small effect on gene expression. It is therefore uncertain whether gene expression and chromatin state drive chromatin organization or whether changes in chromatin organization facilitate cell-type-specific activation of gene expression. Here, using the dorsoventral patterning of the
Drosophila melanogaster
embryo as a model system, we provide evidence for the independence of chromatin organization and dorsoventral gene expression. We define tissue-specific enhancers and link them to expression patterns using single-cell RNA-seq. Surprisingly, despite tissue-specific chromatin states and gene expression, chromatin organization is largely maintained across tissues. Our results indicate that tissue-specific chromatin conformation is not necessary for tissue-specific gene expression but rather acts as a scaffold facilitating gene expression when enhancers become active.
Chromatin conformation is largely independent of dorsoventral gene expression during early embryonic development in
Drosophila
. Despite tissue-specific differences in chromatin state and gene expression, three-dimensional chromatin organization is maintained across tissues.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Animal Genetics and Genomics
/ Animals
/ Animals, Genetically Modified
/ Biomedical and Life Sciences
/ Domains
/ Drosophila melanogaster - cytology
/ Drosophila melanogaster - genetics
/ Drosophila melanogaster - growth & development
/ Drosophila melanogaster - metabolism
/ Drosophila Proteins - genetics
/ Drosophila Proteins - metabolism
/ Embryos
/ Female
/ Gene Expression Regulation, Developmental
/ Genome
/ Genomes
/ High-Throughput Nucleotide Sequencing
/ Insects
/ Male
/ Mutation
/ Proteins
/ Tissues
