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Reconstruction and analysis of a Kluyveromyces marxianus genome-scale metabolic model
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Reconstruction and analysis of a Kluyveromyces marxianus genome-scale metabolic model
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Journal Article
Reconstruction and analysis of a Kluyveromyces marxianus genome-scale metabolic model
2019
Overview
Background
Kluyveromyces marxianus
is a thermotolerant yeast with multiple biotechnological potentials for industrial applications, which can metabolize a broad range of carbon sources, including less conventional sugars like lactose, xylose, arabinose and inulin. These phenotypic traits are sustained even up to 45 °C, what makes it a relevant candidate for industrial biotechnology applications, such as ethanol production. It is therefore of much interest to get more insight into the metabolism of this yeast. Recent studies suggested, that thermotolerance is achieved by reducing the number of growth-determining proteins or suppressing oxidative phosphorylation. Here we aimed to find related factors contributing to the thermotolerance of
K. marxianus
.
Results
Here, we reported the first genome-scale metabolic model of
Kluyveromyces marxianus,
iSM996, using a publicly available
Kluyveromyces lactis
model as template. The model was manually curated and refined to include the missing species-specific metabolic capabilities. The iSM996 model includes 1913 reactions, associated with 996 genes and 1531 metabolites. It performed well to predict the carbon source utilization and growth rates under different growth conditions. Moreover, the model was coupled with transcriptomics data and used to perform simulations at various growth temperatures.
Conclusions
K. marxianus
iSM996 represents a well-annotated metabolic model of thermotolerant yeast, which provides a new insight into theoretical metabolic profiles at different temperatures of
K. marxianus
. This could accelerate the integrative analysis of multi-omics data, leading to model-driven strain design and improvement.
Publisher
BioMed Central,BioMed Central Ltd,BMC
Subject
/ Analysis
/ Analysis and modelling of complex systems
/ Biomass
/ Biomedical and Life Sciences
/ Biosynthetic Pathways - genetics
/ Computational Biology/Bioinformatics
/ Computer Appl. in Life Sciences
/ Constraint-based flux analysis
/ Ethanol
/ Genes
/ Genome-scale metabolic model
/ Genomes
/ Genomics
/ Lactose
/ Proteins
/ Saccharomyces cerevisiae - genetics
