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Molecular, cellular and neurological consequences of infection by the neglected human pathogen Nocardia
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Molecular, cellular and neurological consequences of infection by the neglected human pathogen Nocardia
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Molecular, cellular and neurological consequences of infection by the neglected human pathogen Nocardia
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Journal Article
Molecular, cellular and neurological consequences of infection by the neglected human pathogen Nocardia
2022
Overview
Background
Nocardia
is a facultative intracellular pathogen that infects the lungs and brains of immunocompromised patients with consequences that can be fatal. The incidence of such infections is rising, immunocompetent individuals are also being infected, and there is a need to learn more about this neglected bacterial pathogen and the interaction with its human host.
Results
We have applied dual RNA-seq to assess the global transcriptome changes that occur simultaneously in
Nocardia farcinica
(
N. farcinica
) and infected human epithelial alveolar host cells, and have tested a series of mutants in this in vitro system to identify candidate determinants of virulence. Using a mouse model, we revealed the profiles of inflammation-related factors in the lung after intranasal infection and confirmed that nbtB and nbtS are key virulence genes for
Nocardia
infection in vivo. Regarding the host response to infection, we found that the expression of many histones was dysregulated during the infection of lung cells, indicating that epigenetic modification might play a crucial role in the host during
Nocardia
infection. In our mouse model,
Nocardia
infection led to neurological symptoms and we found that 15 of 22
Nocardia
clinical strains tested could cause obvious PD-like symptoms. Further experiments indicated that
Nocardia
infection could activate microglia and drive M1 microglial polarization, promote iNOS and CXCL-10 production, and cause neuroinflammation in the substantia nigra, all of which may be involved in causing PD-like symptoms. Importantly, the deletion of nbtS in
N. farcinica
completely attenuated the neurological symptoms.
Conclusions
Our data contribute to an in-depth understanding of the characteristics of both the host and
Nocardia
during infection and provide valuable clues for future studies of this neglected human pathogen, especially those addressing the underlying causes of infection-related neurological symptoms.
