Journal Article

Sr-substituted bone cements direct mesenchymal stem cells, osteoblasts and osteoclasts fate

Sprio, Simone, Dapporto, Massimiliano, Montesi, Monica, Panseri, Silvia, Tampieri, Anna

2017

Overview

Strontium-substituted apatitic bone cements enriched with sodium alginate were developed as a potential modulator of bone cells fate. The biological impact of the bone cement were investigated in vitro through the study of the effect of the nanostructured apatitic composition and the doping of strontium on mesenchymal stem cells, pre-osteoblasts and osteoclasts behaviours. Up to 14 days of culture the bone cells viability, proliferation, morphology and gene expression profiles were evaluated. The results showed that different concentrations of strontium were able to evoke a cell-specific response, in fact an inductive effect on mesenchymal stem cells differentiation and pre-osteoblasts proliferation and an inhibitory effect on osteoclasts activity were observed. Moreover, the apatitic structure of the cements provided a biomimetic environment suitable for bone cells growth. Therefore, the combination of biological features of this bone cement makes it as promising biomaterials for tissue regeneration.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Alginic acid / Animals / Biocompatibility / Biological activity / Biology and Life Sciences / Biomaterials / Biomedical materials / Biomimetics / Bone biomaterials / Bone cements / Bone Cements - chemistry / Bone Cements - pharmacology / Bone growth / Bone implants / Bone marrow / Calcium phosphates / Cell culture / Cell Line / Cell proliferation / Cell Proliferation - drug effects / Cell Survival - drug effects / Cement / Composition effects / Councils / Fractures / Gene expression / Health aspects / Hydroxyapatite / Materials Testing / Medicine and Health Sciences / Mesenchymal stem cells / Mesenchymal Stem Cells - cytology / Mesenchymal Stem Cells - metabolism / Mesenchyme / Mice / Osteoblastogenesis / Osteoblasts / Osteoblasts - cytology / Osteoblasts - metabolism / Osteoclastogenesis / Osteoclasts / Osteoclasts (Biology) / Osteoclasts - metabolism / Osteoporosis / Physical Sciences / Physiological aspects / Public health / Regeneration / Science / Sodium / Sodium alginate / Stem cells / Strontium / Strontium - chemistry / Strontium - pharmacology / Substitutes / Tissue engineering / Viability