Journal Article

Transcriptome Analysis Revealed Changes of Multiple Genes Involved in Haliotis discus hannai Innate Immunity during Vibrio parahemolyticus Infection

Nam, Bo-Hye, Markkandan, Kesavan, Jung, Ho-jin, Kim, Dong-Gyun, Yoo, Seung-il, Moon, Ji-Young, Jung, Myunghee, Shin, Younhee, Subramaniyam, Sathiyamoorthy, Kim, Young-Ok, Park, Jun-hyung, An, Cheul Min

2016

Overview

Abalone (Haliotis discus hannai) is one of the most valuable marine aquatic species in Korea, Japan and China. Tremendous exposure to bacterial infection is common in aquaculture environment, especially by Vibrio sp. infections. It's therefore necessary and urgent to understand the mechanism of H. discus hannai host defense against Vibrio parahemolyticus infection. However studies on its immune system are hindered by the lack of genomic resources. In the present study, we sequenced the transcriptome of control and bacterial challenged H. discus hannai tissues. Totally, 138 MB of reference transcriptome were obtained from de novo assembly of 34 GB clean bases from ten different libraries and annotated with the biological terms (GO and KEGG). A total of 10,575 transcripts exhibiting the differentially expression at least one pair of comparison and the functional annotations highlight genes related to immune response, cell adhesion, immune regulators, redox molecules and mitochondrial coding genes. Mostly, these groups of genes were dominated in hemocytes compared to other tissues. This work is a prerequisite for the identification of those physiological traits controlling H. discus hannai ability to survive against Vibrio infection.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Abalones / Analysis / Animal Diseases - microbiology / Animals / Annotations / Aquaculture / Bacteria / Bacterial diseases / Bacterial infections / Biochemistry / Biology and Life Sciences / Biotechnology / Cell adhesion / Cell adhesion molecules / Cloning / Crassostrea virginica / Diseases and pests / Fish / Fisheries / Fishing / Gastropoda - genetics / Gastropoda - immunology / Gastropoda - microbiology / Gene expression / Gene Expression Profiling / Gene Expression Regulation / Genes / Genetic aspects / Genome, Mitochondrial / Genomes / Haliotis / Haliotis discus discus / Haliotis discus hannai / Haliotis rufescens / Health aspects / Hemocytes / Host-Pathogen Interactions - genetics / Host-Pathogen Interactions - immunology / Identification / Immune response / Immune system / Immunity / Immunity, Innate - genetics / Immunology / Infections / Innate immunity / Lectins / Medicine and Health Sciences / Mitochondria / Physiological aspects / Physiology / Proteins / R&D / Real-Time Polymerase Chain Reaction / Regulators / Reproducibility of Results / Research & development / Research and Analysis Methods / Stability / Tissues / Vibrio / Vibrio Infections - genetics / Vibrio Infections - immunology / Vibrio Infections - veterinary / Vibrio parahaemolyticus / Water-borne diseases / Waterborne diseases