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MicroRNA miR-30 family regulates non-attachment growth of breast cancer cells
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MicroRNA miR-30 family regulates non-attachment growth of breast cancer cells
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Journal Article
MicroRNA miR-30 family regulates non-attachment growth of breast cancer cells
2013
Overview
Background
A subset of breast cancer cells displays increased ability to self-renew and reproduce breast cancer heterogeneity. The characterization of these so-called putative breast tumor-initiating cells (BT-ICs) may open the road for novel therapeutic strategies. As microRNAs (miRNAs) control developmental programs in stem cells, BT-ICs may also rely on specific miRNA profiles for their sustained activity. To explore the notion that miRNAs may have a role in sustaining BT-ICs, we performed a comprehensive profiling of miRNA expression in a model of putative BT-ICs enriched by non-attachment growth conditions.
Results
We found breast cancer cells grown under non-attachment conditions display a unique pattern of miRNA expression, highlighted by a marked low expression of miR-30 family members relative to parental cells. We further show that miR-30a regulates non-attachment growth. A target screening revealed that miR-30 family redundantly modulates the expression of apoptosis and proliferation-related genes. At least one of these targets, the anti-apoptotic protein AVEN, was able to partially revert the effect of miR-30a overexpression. Finally, overexpression of miR-30a in vivo was associated with reduced breast tumor progression.
Conclusions
miR30-family regulates the growth of breast cancer cells in non-attachment conditions. This is the first analysis of target prediction in a whole family of microRNAs potentially involved in survival of putative BT-ICs.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V
Subject
Adaptor Proteins, Signal Transducing - metabolism
/ Animal Genetics and Genomics
/ Animals
/ Apoptosis Regulatory Proteins - metabolism
/ Biomedical and Life Sciences
/ Breast Neoplasms - pathology
/ Cell Transformation, Neoplastic
/ Female
/ Growth
/ Membrane Proteins - metabolism
/ Mice
/ Microbial Genetics and Genomics
/ MicroRNA
/ miRNA
/ Neoplastic Stem Cells - metabolism
/ Neoplastic Stem Cells - pathology
/ Ontology
/ Tumors
