Journal Article

Assessing the relationship between the rumen microbiota and feed efficiency in Nellore steers

Lopes, Déborah Romaskevis Gomes, La Reau, Alex J., de Oliveira Mendes, Tiago Antônio, Bento, Cláudia Braga Pereira, Mercadante, Maria Eugênia Zerlotti, Mantovani, Hilário Cuquetto, Chaves, Ibrahim Zuniga, Bonilha, Sarah Figueiredo Martins, Suen, Garret, Detmann, Edenio, de Souza Duarte, Márcio

2021

Overview

Background Ruminants rely upon a complex community of microbes in their rumen to convert host-indigestible feed into nutrients. However, little is known about the association between the rumen microbiota and feed efficiency traits in Nellore ( Bos indicus ) cattle, a breed of major economic importance to the global beef market. Here, we compare the composition of the bacterial, archaeal and fungal communities in the rumen of Nellore steers with high and low feed efficiency (FE) phenotypes, as measured by residual feed intake (RFI). Results The Firmicutes to Bacteroidetes ratio was significantly higher ( P  < 0.05) in positive-RFI steers (p-RFI, low feed efficiency) than in negative-RFI (n-RFI, high feed efficiency) steers. The differences in bacterial composition from steers with high and low FE were mainly associated with members of the families Lachnospiraceae, Ruminococcaceae and Christensenellaceae, as well as the genus Prevotella . Archaeal community richness was lower ( P  < 0.05) in p-RFI than in n-RFI steers and the genus Methanobrevibacter was either increased or exclusive of p-RFI steers. The fungal genus Buwchfawromyces was more abundant in the rumen solid fraction of n-RFI steers ( P  < 0.05) and a highly abundant OTU belonging to the genus Piromyces was also increased in the rumen microbiota of high-efficiency steers. However, analysis of rumen fermentation variables and functional predictions indicated similar metabolic outputs for the microbiota of distinct FE groups. Conclusions Our results demonstrate that differences in the ruminal microbiota of high and low FE Nellore steers comprise specific taxa from the bacterial, archaeal and fungal communities. Biomarker OTUs belonging to the genus Piromyces were identified in animals showing high feed efficiency, whereas among archaea, Methanobrevibacter was associated with steers classified as p-RFI. The identification of specific RFI-associated microorganisms in Nellore steers could guide further studies targeting the isolation and functional characterization of rumen microbes potentially important for the energy-harvesting efficiency of ruminants.

• Publisher: BioMed Central,Springer Nature B.V,BMC
• Subject: 16S rRNA gene / Agriculture / Animal Genetics and Genomics / Animal Physiology / Animals / Archaea / Automation / Bacteria / Bacteroidetes / beef / Beef cattle / Biomarkers / Biomedical and Life Sciences / Biotechnology / Bos taurus indicus / Composition / Diet / Economic importance / Efficiency / Energy conversion efficiency / Energy harvesting / Fatty acids / Feed conversion / Feed efficiency / feed intake / Feeds / Fermentation / Firmicutes / Food Science / Fungi / genus / ITS1 region / Lachnospiraceae / Life Sciences / markets / Mathematical analysis / Metabolism / Metabolites / Methanobrevibacter / Microbiota / Microorganisms / Nellore / Next-generation sequencing / Nutrients / Phenotypes / Piromyces / Prevotella / Regression analysis / RFI / Rumen / Rumen microbiota / Ruminococcaceae / zebu