Journal Article

Bone Marrow Stromal Cells Generate Muscle Cells and Repair Muscle Degeneration

Nabeshima, Yo-ichi, Ishikawa, Hiroto, Itokazu, Yutaka, Hoshino, Mikio, Yoshihara, Tomoyuki, Dezawa, Mari, Takeda, Shin-ichi, Ide, Chizuka

2005

Overview

Bone marrow stromal cells (MSCs) have great potential as therapeutic agents. We report a method for inducing skeletal muscle lineage cells from human and rat general adherent MSCs with an efficiency of 89%. Induced cells differentiated into muscle fibers upon transplantation into degenerated muscles of rats and mdx-nude mice. The induced population contained Pax7-positive cells that contributed to subsequent regeneration of muscle upon repetitive damage without additional transplantation of cells. These MSCs represent a more ready supply of myogenic cells than do the rare myogenic stem cells normally found in muscle and bone marrow.

• Publisher: American Association for the Advancement of Science,The American Association for the Advancement of Science
• Subject: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy / Animals / Applied cell therapy and gene therapy / Artificial satellites / Biological and medical sciences / Bone marrow / Bone Marrow Cells - cytology / Bone Marrow Cells - physiology / Bone Marrow Transplantation / Cell Differentiation / Cell Fusion / Cell Lineage / Cell lines / Cell Proliferation / Cell Separation / Cell transplantation / Cells / Cells, Cultured / Colforsin - pharmacology / Dystonia / Ethics / Fibroblast Growth Factor 2 - pharmacology / Gene Expression Profiling / Hematopoietic stem cell transplantation / Hematopoietic stem cells / Homeodomain Proteins - analysis / Humans / Individualized Instruction / Logical Thinking / Medical disorders / Medical sciences / Mice / Mice, Inbred mdx / Mice, Nude / Muscle Cells - cytology / Muscle Development - genetics / Muscle diseases / muscle fibers / Muscle Fibers, Skeletal - cytology / Muscle Proteins - analysis / Muscle, Skeletal - cytology / Muscles / Muscular diseases / Muscular Diseases - therapy / Muscular Dystrophy, Duchenne - therapy / Muscular system / myocytes / Neuregulins - pharmacology / Neuroglia / Parkinson disease / Parkinson's disease / PAX7 Transcription Factor / Platelet-Derived Growth Factor - pharmacology / Rats / Regeneration / Satellite Cells, Skeletal Muscle - cytology / Satellite Cells, Skeletal Muscle - physiology / skeletal muscle / Skeletal muscle satellite cells / Stem cell transplantation / Stem cells / Stem Cells - cytology / Stem Cells - physiology / Stromal cells / Stromal Cells - cytology / Stromal Cells - physiology / Stromal Cells - transplantation / Transfection / Transfusions. Complications. Transfusion reactions. Cell and gene therapy / Transplantation / Transplants & implants