Journal Article

Vaccination to Conserved Influenza Antigens in Mice Using a Novel Simian Adenovirus Vector, PanAd3, Derived from the Bonobo Pan paniscus

Vitelli, Alessandra, Price, Graeme E., Misplon, Julia A., Nicosia, Alfredo, Pierantoni, Angiolo, Quirion, Mary R., Soboleski, Mark R., Epstein, Suzanne L., Grabowska, Agnieszka K., Colloca, Stefano, Ammendola, Virginia, Lo, Chia-Yun, Cortese, Riccardo

2013

Overview

Among approximately 1000 adenoviruses from chimpanzees and bonobos studied recently, the Pan Adenovirus type 3 (PanAd3, isolated from a bonobo, Pan paniscus) has one of the best profiles for a vaccine vector, combining potent transgene immunogenicity with minimal pre-existing immunity in the human population. In this study, we inserted into a replication defective PanAd3 a transgene expressing a fusion protein of conserved influenza antigens nucleoprotein (NP) and matrix 1 (M1). We then studied antibody and T cell responses as well as protection from challenge infection in a mouse model. A single intranasal administration of PanAd3-NPM1 vaccine induced strong antibody and T cell responses, and protected against high dose lethal influenza virus challenge. Thus PanAd3 is a promising candidate vector for vaccines, including universal influenza vaccines.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Adenoviruses / Adenoviruses, Human - immunology / Adenoviruses, Simian - genetics / Adenoviruses, Simian - immunology / Amino Acid Sequence / Animals / Antibodies, Neutralizing - immunology / Antibodies, Viral - blood / Antibodies, Viral - immunology / Antigens / Antigens, Viral - genetics / Antigens, Viral - immunology / Avian flu / Biology / Chimpanzees / Combination drug therapy / Cross Reactions - immunology / Defects / Female / Food / Fusion protein / Gene Expression / Gene therapy / Genetic Vectors - administration & dosage / Genetic Vectors - genetics / Genetic Vectors - immunology / Human populations / Humans / Immunity / Immunity, Mucosal / Immunogenicity / Immunoglobulins / Influenza / Influenza A Virus, H1N1 Subtype - genetics / Influenza A Virus, H1N1 Subtype - immunology / Influenza vaccines / Influenza Vaccines - administration & dosage / Influenza Vaccines - immunology / Influenza viruses / Intranasal administration / Lymphocytes / Lymphocytes T / Medical research / Medicine / Mice / Molecular Sequence Data / Orthomyxoviridae Infections - prevention & control / Pan paniscus / Population studies / Proteins / Recombinant Fusion Proteins - chemistry / Recombinant Fusion Proteins - genetics / Recombinant Fusion Proteins - immunology / RNA-Binding Proteins - chemistry / RNA-Binding Proteins - genetics / RNA-Binding Proteins - immunology / T cell receptors / T cells / T-Lymphocytes - immunology / Vaccination / Vaccines / Vectors (Biology) / Viral Core Proteins - chemistry / Viral Core Proteins - genetics / Viral Core Proteins - immunology / Viral Matrix Proteins - chemistry / Viral Matrix Proteins - genetics / Viral Matrix Proteins - immunology / Viral proteins / Viruses