Journal Article

New Metrics for Evaluating Viral Respiratory Pathogenesis

Ferris, Martin T., Gralinski, Lisa E., Baric, Ralph S., Menachery, Vineet D.

2015

Overview

Viral pathogenesis studies in mice have relied on markers of severe systemic disease, rather than clinically relevant measures, to evaluate respiratory virus infection; thus confounding connections to human disease. Here, whole-body plethysmography was used to directly measure changes in pulmonary function during two respiratory viral infections. This methodology closely tracked with traditional pathogenesis metrics, distinguished both virus- and dose-specific responses, and identified long-term respiratory changes following both SARS-CoV and Influenza A Virus infection. Together, the work highlights the utility of examining respiratory function following infection in order to fully understand viral pathogenesis.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Analysis / Animals / Bacterial infections / Chlorocebus aethiops / Disease / Disease Models, Animal / Dogs / Epidemiology / Health aspects / Infection / Infections / Influenza / Influenza A / Influenza A Virus, H1N1 Subtype / Life assessment / Lung - pathology / Lung - virology / Madin Darby Canine Kidney Cells / Mice / Mice, Inbred C57BL / Mortality / Orthomyxoviridae Infections - physiopathology / Pathogenesis / Plethysmography / Plethysmography, Whole Body / Predictive Value of Tests / Pulmonary functions / Respiratory diseases / Respiratory function / Respiratory syncytial virus / Severe Acute Respiratory Syndrome - physiopathology / Severe acute respiratory syndrome-related coronavirus / Vero Cells / Viral infections / Viral Load / Virus Replication - physiology / Viruses