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Rv1460, a SufR homologue, is a repressor of the suf operon in Mycobacterium tuberculosis
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Rv1460, a SufR homologue, is a repressor of the suf operon in Mycobacterium tuberculosis
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Rv1460, a SufR homologue, is a repressor of the suf operon in Mycobacterium tuberculosis
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Journal Article
Rv1460, a SufR homologue, is a repressor of the suf operon in Mycobacterium tuberculosis
2018
Overview
Iron-sulphur (Fe-S) clusters are ubiquitous co-factors which require multi-protein systems for their synthesis. In Mycobacterium tuberculosis, the Rv1460-Rv1461-Rv1462-Rv1463-csd-Rv1465-Rv1466 operon (suf operon) encodes the primary Fe-S cluster biogenesis system. The first gene in this operon, Rv1460, shares homology with the cyanobacterial SufR, which functions as a transcriptional repressor of the sufBCDS operon. Rv1460's function in M. tuberculosis has however not been determined. In this study, we demonstrate that M. tuberculosis mutants lacking a functional Rv1460 protein are impaired for growth under standard culture conditions. Elevated expression of Rv1460 and Rv1461 was observed in the mutant, implicating Rv1460 in the regulation of the suf operon. Binding of an Fe-S cluster to purified recombinant Rv1460 was confirmed by UV-visible spectroscopy and circular dichroism. Furthermore, three conserved cysteine residues, C203, C216 and C244, proposed to provide ligands for the coordination of an Fe-S cluster, were shown to be required for the function of Rv1460 in M. tuberculosis. Rv1460 therefore seems to be functionally analogous to cyanobacterial SufR.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Bacteria
/ Bacterial Proteins - chemistry
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Clusters
/ Councils
/ Cysteine
/ DNA
/ E coli
/ Enzymes
/ Genomes
/ Homology
/ Humans
/ Iron
/ Iron-Sulfur Proteins - chemistry
/ Iron-Sulfur Proteins - genetics
/ Iron-Sulfur Proteins - metabolism
/ Mutants
/ Mutation
/ Mycobacterium tuberculosis - genetics
/ Mycobacterium tuberculosis - growth & development
/ Mycobacterium tuberculosis - metabolism
/ Operon
/ Proteins
/ Recombinant Proteins - chemistry
/ Recombinant Proteins - genetics
/ Recombinant Proteins - metabolism
/ Repressor Proteins - chemistry
/ Repressor Proteins - genetics
/ Repressor Proteins - metabolism
/ Sulfur
