Journal Article

The Protein Precursors of Peptides That Affect the Mechanics of Connective Tissue and/or Muscle in the Echinoderm Apostichopus japonicus

Elphick, Maurice R.

2012

Overview

Peptides that cause muscle relaxation or contraction or that modulate electrically-induced muscle contraction have been discovered in the sea cucumber Apostichopus japonicus (Phylum Echinodermata; Class Holothuroidea). By analysing transcriptome sequence data, here the protein precursors of six of these myoactive peptides (the SALMFamides Sticho-MFamide-1 and -2, NGIWYamide, stichopin, GN-19 and GLRFA) have been identified, providing novel insights on neuropeptide and endocrine-type signalling systems in echinoderms. The A. japonicus SALMFamide precursor comprises eight putative neuropeptides including both L-type and F-type SALMFamides, which contrasts with previous findings from the sea urchin Strongylocentrotus purpuratus where L-type and F-type SALMFamides are encoded by different genes. The NGIWYamide precursor contains five copies of NGIWYamide but, unlike other NG peptide-type neuropeptide precursors in deuterostomian invertebrates, the NGIWYamide precursor does not have a C-terminal neurophysin domain, indicating loss of this character in holothurians. NGIWYamide was originally discovered as a muscle contractant, but it also causes stiffening of mutable connective tissue in the body wall of A. japonicus, whilst holokinins (PLGYMFR and derivative peptides) cause softening of the body wall. However, the mechanisms by which these peptides affect the stiffness of body wall connective tissue are unknown. Interestingly, analysis of the A. japonicus transcriptome reveals that the only protein containing the holokinin sequence PLGYMFR is an alpha-5 type collagen. This suggests that proteolysis of collagen may generate peptides (holokinins) that affect body wall stiffness in sea cucumbers, providing a novel perspective on mechanisms of mutable connective tissue in echinoderms.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Amino Acid Sequence / Analysis / Animal behavior / Animals / Apostichopus japonicus / Base Sequence / Biology / Biomechanical Phenomena / Body wall / Coding / Collagen / Connective Tissue - metabolism / Connective tissues / Data processing / Echinodermata / Echinoderms / Echinoidea / Gene expression / Genetic aspects / Holothuria glaberrima / Holothuroidea / Invertebrates / Molecular Sequence Data / Muscle contraction / Muscles / Muscles - metabolism / Nervous system / Neuropeptides / Neuropeptides - chemistry / Neuropeptides - genetics / Neurophysin / Peptides / Peptides - chemistry / Peptides - genetics / Peptides - isolation & purification / Peptides - metabolism / Physiological aspects / Physiology / Precursors / Protein Precursors - chemistry / Protein Precursors - genetics / Protein Precursors - metabolism / Proteins / Proteolysis / Signalling systems / Stichopus - metabolism / Stichopus japonicus / Stiffening / Stiffness