Journal Article

MicroRNA-503 Acts as a Tumor Suppressor in Osteosarcoma by Targeting L1CAM

Jiao, Zhijian, Shao, Ming, Li, Guojun, Chong, Yang, Zhang, Jie, Li, Chao, Guo, Xinzhen, Zhang, Shiqian

2014

Overview

Deregulated microRNAs and their roles in tumorigenesis have attracted much attention in recent years. Although miR-503 was shown to be important in tumorigenesis, its role in osteosarcoma remains unknown. In this study, we focused on the expression and mechanisms of miR-503 in osteosarcoma development. We found that miR-503 was down-regulated in osteosarcoma cell lines and primary tumor samples, and the restoration of miR-503 reduced cell proliferation, migration and invasion. Low level of miR-503 in patients with osteosarcoma was associated with considerably shortened disease-free survival. Furthermore, bioinformatic prediction and experimental validation revealed that the anti-tumor effect of miR-503 was probably exerted through targeting and repressing of L1CAM expression. L1CAM was up-regulated in osteosarcoma cell lines and primary tumor samples and the expression level of L1CAM were negatively correlated with miR-503 levels in osteosarcoma tissues. Collectively, our data identify the important roles of miR-503 in osteosarcoma pathogenesis, indicating its potential application in cancer therapy.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Adolescent / Anticancer properties / Base Sequence / Biocompatibility / Biology and Life Sciences / Biotechnology / Bone cancer / Bone surgery / Cancer / Cell adhesion & migration / Cell growth / Cell Line, Tumor / Cell migration / Cell Movement - genetics / Cell Proliferation / Child / Data processing / Deregulation / Down-Regulation - genetics / Female / Gastric cancer / Gene Expression Regulation, Neoplastic / Genes, Tumor Suppressor / HEK293 Cells / Humans / Kinases / Liver cancer / Low level / Male / Medicine and Health Sciences / Melanoma / Metastasis / MicroRNA / MicroRNAs / MicroRNAs - genetics / MicroRNAs - metabolism / miRNA / Molecular Sequence Data / Motility / Neoplasm Invasiveness / Neural Cell Adhesion Molecule L1 - metabolism / Orthopedics / Osteosarcoma / Osteosarcoma - genetics / Osteosarcoma - pathology / Pathogenesis / Research and Analysis Methods / Restoration / Ribonucleic acid / RNA / Sarcoma / Stomach cancer / Tissues / Tumor cell lines / Tumor suppressor genes / Tumorigenesis / Tumors