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HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer
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HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer
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Journal Article
HHIP overexpression inhibits the proliferation, migration and invasion of non-small cell lung cancer
2019
Overview
The primary purpose of this study is to investigate the effect of hedgehog-interacting protein (HHIP) overexpression on the proliferation, migration and invasion of non-small cell lung cancer (NSCLC).
Firstly, HHIP gene expression data of NSCLC tissues and normal tissues were obtained from GSE18842/GSE19804/GSE43458 databases of the Gene Expression Omnibus (GEO) database and then validated by TCGA NSCLC database in a cohort of 1027 cases of NSCLC patients and 108 cases of normal people. A chi-square test was used to analyze the relationship between HHIP expression and clinicopathological characteristics of NSCLC. The expression levels of HHIP in NSCLC cells were detected by quantitative-real time PCR. The function of HHIP was investigated by a series of in vitro assays. CCK-8, wounding healing, Transwell invasion assay were utilized to explore the mechanisms of HHIP.
HHIP mRNA were significantly down-regulated in NSCLC in three GEO databases and TCGA database (P<0.05). This result was confirmed in NSCLC cell lines by qRT-PCR analysis, its expression in normal NSCLC cell line BEAS-2B was significantly higher than that in NSCLC cells. Chi-square test results showed that the low expression of HHIP was correlated with gender, cancer type, TNM stage and tumor size. Functional experimental results showed that over-expressing HHIP significantly decreased the ability of cell proliferation, migration and invasion in NSCLC cells (P<0.05).
Overall, the above results indicated that HHIP could regulate proliferation, migration and invasion, and could be used as a judging criterion for identifying NSCLC classification and stage.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Carcinoma, Non-Small-Cell Lung - metabolism
/ Carcinoma, Non-Small-Cell Lung - pathology
/ Carrier Proteins - metabolism
/ Female
/ Genes
/ Hedgehog-interacting protein
/ Humans
/ Male
/ Medicine
/ Medicine and Health Sciences
/ Membrane Glycoproteins - genetics
/ Membrane Glycoproteins - metabolism
/ Non-small cell lung carcinoma
/ Oncology
/ Proteins
/ RNA
/ Tumors
/ Wounding
