Journal Article

Bisphenol A in Oocytes Leads to Growth Suppression and Altered Stress Performance in Juvenile Rainbow Trout

Leatherland, John F., Vijayan, Mathilakath M., Aluru, Neelakanteswar

2010

Overview

Bisphenol A (BPA), used in the manufacture of plastics, is ubiquitously distributed in the aquatic environment. However, the effect of maternal transfer of these xenobiotics on embryonic development and growth is poorly understood in fish. We tested the hypothesis that BPA in eggs, mimicking maternal transfer, impact development, growth and stress performance in juveniles of rainbow trout (Oncorhynchus mykiss). Trout oocytes were exposed to 0, 30 and 100 microg.mL(-1) BPA for 3 h in ovarian fluid, followed by fertilization. The embryos were maintained in clean water and sampled temporally over 156-days post-fertilization (dpf), and juveniles were sampled at 400-dpf. The egg BPA levels declined steadily after exposure and were undetectable after 21- dpf. Oocyte exposure to BPA led to a delay in hatching and yolk absorption and a consistently lower body mass over 152-dpf. The growth impairment, especially in the high BPA group, correlated with higher growth hormone (GH) content and lower GH receptors gene expression. Also, mRNA abundances of insulin-like growth factors (IGF-1 and IGF-2) and their receptors were suppressed in the BPA treated groups. The juvenile fish grown from the BPA-enriched eggs had lower body mass and showed perturbations in plasma cortisol and glucose response to an acute stressor. BPA accumulation in eggs, prior to fertilization, leads to hatching delays, growth suppression and altered stress response in juvenile trout. The somatotropic axis appears to be a key target for BPA impact during early embryogenesis, leading to long term growth and stress performance defects in fish.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Aging - drug effects / Analysis / Animals / Aquatic environment / Benzhydryl Compounds / Bisphenol A / Body mass / Cortisol / Eggs / Eggs (Food) / Embryo, Nonmammalian - drug effects / Embryo, Nonmammalian - metabolism / Embryogenesis / Embryonic development / Embryonic Development - drug effects / Embryonic Development - genetics / Embryonic growth stage / Embryos / Environmental effects / Exposure / Fertilization / Fish / Fish eggs / Fishes / Gene expression / Gene Expression Regulation, Developmental - drug effects / Glucocorticoids / Growth / Growth factors / Growth hormone / Growth Hormone - genetics / Growth Hormone - metabolism / Growth hormones / Hatching / Insulin / Insulin-like growth factor I / Juveniles / Marine and Aquatic Sciences / Maternal transfer / Mimicry / Oncorhynchus mykiss / Oncorhynchus mykiss - embryology / Oncorhynchus mykiss - genetics / Oncorhynchus mykiss - growth & development / Oocytes / Oocytes - drug effects / Oocytes - metabolism / Phenols / Phenols (Class of compounds) / Phenols - pharmacology / Phenotype / Physiology/Endocrinology / Physiology/Integrative Physiology / Polymers / Receptors / Receptors, Somatomedin - genetics / Receptors, Somatomedin - metabolism / Receptors, Somatotropin - genetics / Receptors, Somatotropin - metabolism / RNA / Rodents / Salmon / Salvelinus alpinus / Somatomedins - genetics / Somatomedins - metabolism / Somatotropin / Stress / Stress, Physiological - drug effects / Stresses / Survival Analysis / Trout / Vitellogenins - genetics / Vitellogenins - metabolism / Xenobiotics / Yolk