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Temperature of a Dengue Rapid Diagnostic Test under Tropical Climatic Conditions: A Follow Up Study
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Temperature of a Dengue Rapid Diagnostic Test under Tropical Climatic Conditions: A Follow Up Study
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Journal Article
Temperature of a Dengue Rapid Diagnostic Test under Tropical Climatic Conditions: A Follow Up Study
2017
Overview
The Dengue Duo Rapid Diagnostic Test (SD Dengue RDT) has good specificity and sensitivity for dengue diagnosis in rural tropical areas. In a previous study, using four control sera, we demonstrated that that the diagnostic accuracy of these RDTs remains stable after long-term storage at high temperatures. We extended this study by testing sera from 119 febrile patients collected between July-November 2012 at Salavan Provincial Hospital (southern Laos) with RDTs stored for 6 months at 4°C, 35° and in a hut (miniature traditional house) at Lao ambient temperatures. The dengue NS1 antigen results from RDTs stored at 35°C and in the hut demonstrated 100% agreement with those stored at 4°C. However, lower positive percent agreements, with broad 95%CI, were observed for the tests: IgM, 60% (14.7-94.7) and 40% (5.3-85.3) for RDTs store at 35°C and in the hut, compared to those stored at 4°C, respectively. This study strenghtens the evidence of the robustness of the NS1 antigen detection RDT for the diagnosis of dengue after storage at tropical temperatures.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Antibodies, Viral - chemistry
/ Antibodies, Viral - immunology
/ Antigens
/ Dengue
/ Dengue Virus - isolation & purification
/ Dengue Virus - pathogenicity
/ Diagnostic Tests, Routine - standards
/ Female
/ Humans
/ Laos
/ Male
/ Medicine
/ Medicine and Health Sciences
/ Reagent Kits, Diagnostic - standards
/ Research and Analysis Methods
/ Storage
