Journal Article

Comparison of two portable clinical analyzers to one stationary analyzer for the determination of blood gas partial pressures and blood electrolyte concentrations in horses

Kirsch, Katharina, Detilleux, Johann, Sandersen, Charlotte, Serteyn, Didier

2019

Overview

Portable blood gas analyzers are used to facilitate diagnosis and treatment of disorders related to disturbances of acid-base and electrolyte balance in the ambulatory care of equine patients. The aim of this study was to determine whether 2 portable analyzers produce results in agreement with a stationary analyzer. Blood samples from 23 horses hospitalized for various medical reasons were included in this prospective study. Blood gas analysis and electrolyte concentrations measured by the portable analyzers VetStat and epoc were compared to those produced by the cobas b 123 analyzer via concordance analysis, Passing-Bablok regression and Bland-Altman analysis. Limits of agreement indicated relevant bias between the VetStat and cobas b 123 for partial pressure of oxygen (pO2; 27.5-33.8 mmHg), sodium ([Na+]; 4.3-21.6 mmol/L) and chloride concentration ([Cl-]; 0.3-7.9 mmol/L) and between the epoc and cobas b 123 for pH (0.070-0.022), partial pressure of carbon dioxide (pCO2; 3.6-7.3 mmHg), pO2 (36.2-32.7 mmHg) and [Na+] (0.38.1 mmol/L). The VetStat analyzer yielded results that were in agreement with the cobas b 123 analyzer for determination of pH, pCO2, bicarbonate ([HCO3-]) and potassium concentration [K+], while the epoc analyzer achieved acceptable agreement for [HCO3-] and [K+]. The VetStat analyzer may be useful in performing blood gas analysis in equine samples but analysis of [Na+], [Cl-] and pO2 should be interpreted with caution. The epoc delivered reliable results for [HCO3-] and [K+], while results for pH, pCO2, pO2 and [Na+] should be interpreted with caution.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Acids / Ambulatory care / Analysis / Analytical instruments / Analyzers / Animals / Bicarbonate / Bicarbonates / Bicarbonates - blood / Biology and Life Sciences / Blood / Blood gas analysis / Blood Gas Analysis - instrumentation / Blood Gas Analysis - statistics & numerical data / Blood gas monitors / Blood pressure / Blood tests / Calibration / Carbon dioxide / Carbon Dioxide - blood / Carbonates / Chlorides / Chlorides - blood / Comparative analysis / Electrodes / Electrolyte balance / Electrolytes / Electrolytes - blood / Emergency medical services / Female / Gas analysis / Gas analyzers / Health aspects / Hematology / Hemoglobin / Horses / Horses - blood / Hydrogen-Ion Concentration / Laboratories / Life sciences / Male / Medical research / Medicine and Health Sciences / Methods / Médecine vétérinaire & santé animale / Oxygen / Oxygen - blood / Partial Pressure / pH effects / Physical Sciences / Point-of-Care Systems / Portability / Potassium / Potassium - blood / Pressure / Prospective Studies / Quantitative genetics / Regression analysis / Reproducibility of Results / Research and Analysis Methods / Sciences du vivant / Sodium / Sodium - blood / Studies / Veterinary medicine / Veterinary medicine & animal health