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Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
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Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
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Journal Article
Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
2020
Overview
Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in
ZmALS1
and
ZmALS2
. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.
Publisher
BioMed Central,Springer Nature B.V,BMC
Subject
Acetolactate Synthase - genetics
/ ALS
/ Animal Genetics and Genomics
/ Biomedical and Life Sciences
/ Cloning
/ corn
/ CRISPR
/ DNA
/ Gene Editing - statistics & numerical data
/ genetically modified organisms
/ genome
/ Genomes
/ Harbors
/ Maize
/ Microbial Genetics and Genomics
/ Mutagenesis, Site-Directed - methods
/ Mutation
/ PE2
/ pegRNA
/ Plants, Genetically Modified
/ rice
/ RNA, Guide, CRISPR-Cas Systems - genetics
