Journal Article

A first-in-human study investigating biodistribution, safety and recommended dose of a new radiolabeled MAb targeting FZD10 in metastatic synovial sarcoma patients

Iwao-Fukukawa, Chicaco, Italiano, Antoine, Katagiri, Toyomasa, Kryza, David, Desuzinges, Claude, Pérol, David, Blay, Jean-Yves, Giraudet, Anne-Laure, Gilles-Afchain, Laurence, Sarrut, David, Halty, Adrien, Chabaud, Sylvie, Alberti, Laurent, Cropet, Claire, Berge-Montamat, Sandrine, Garin, Gwenaelle, Baconnier, Simon, Badel, Jean-Noël, Nakamura, Yusuke, Cassier, Philippe Alexandre, Clapisson, Gilles, Mori, Masaharu, Tsunoda, Takuya

2018

Overview

Background Synovial Sarcomas (SS) are rare tumors occurring predominantly in adolescent and young adults with a dismal prognosis in advanced phases. We report a first-in-human phase I of monoclonal antibody (OTSA-101) targeting FZD10 , overexpressed in most SS but not present in normal tissues, labelled with radioisotopes and used as a molecular vehicle to specifically deliver radiation to FZD10 expressing SS lesions. Methods Patients with progressive advanced SS were included. In the first step of this trial, OTSA-101 in vivo bio-distribution and lesions uptake were evaluated by repeated whole body planar and SPECT-CT scintigraphies from H1 till H144 after IV injection of 187 MBq of 111 In-OTSA-101. A 2D dosimetry study also evaluated the liver absorbed dose when using 90 Y-OTSA-101. In the second step, those patients with significant tumor uptake were randomized between 370 MBq (Arm A) and 1110 MBq (Arm B) of 90 Y-OTSA-101 for radionuclide therapy. Results From January 2012 to June 2015, 20 pts. (median age 43 years [21–67]) with advanced SS were enrolled. Even though 111 In-OTSA-101 liver uptake appeared to be intense, estimated absorbed liver dose was less than 20 Gy for each patient. Tracer intensity was greater than mediastinum in 10 patients consistent with sufficient tumor uptake to proceed to treatment with 90 Y-OTSA-101: 8 were randomized (Arm A: 3 patients and Arm B: 5 patients) and 2 were not randomized due to worsening PS. The most common Grade ≥ 3 AEs were reversible hematological disorders, which were more frequent in Arm B. No objective response was observed. Best response was stable disease in 3/8 patients lasting up to 21 weeks for 1 patient. Conclusions Radioimmunotherapy targeting FZD10 is feasible in SS patients as all patients presented at least one lesion with 111 In-OTSA-101 uptake. Tumor uptake was heterogeneous but sufficient to select 50% of pts. for 90 Y-OTSA-101 treatment. The recommended activity for further clinical investigations is 1110 MBq of 90 Y-OTSA-101. However, because of hematological toxicity, less energetic particle emitter radioisopotes such as Lutetium 177 may be a better option to wider the therapeutic index. Trial registration The study was registered on the NCT01469975 website with a registration code NCT01469975 on November the third, 2011.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Adult / Aged / Analysis / Androgens / Antibodies, Monoclonal - pharmacology / Antibodies, Monoclonal - therapeutic use / Antigens / Antineoplastic Agents, Immunological - pharmacology / Antineoplastic Agents, Immunological - therapeutic use / Biomedical and Life Sciences / Biomedicine / Cancer / Cancer Research / Cancer therapies / Care and treatment / Clinical trials / Cytotoxicity / Diagnosis / Dose-Response Relationship, Drug / Dosimetry / Female / First-in-human trial / Frizzled Receptors - antagonists & inhibitors / Gene expression / Health Promotion and Disease Prevention / Humans / Indium / Life Sciences / Liver / Male / Mediastinum / Medical imaging / Medical Physics / Medicine/Public Health / Metastases / Metastasis / Middle Aged / Monoclonal antibodies / Monte Carlo simulation / Oncology / Patients / Physics / post-genomic analysis and emerging technologies / Prostate cancer / Radiation therapy / Radioimmunotherapy / Radioimmunotherapy - methods / Radioisotopes / Research Article / Sarcoma / Sarcoma, Synovial - radiotherapy / Single photon emission computed tomography / Surgical Oncology / Synovial sarcoma / Systems biology / Theranostic / Tissue Distribution / Tomography / Toxicity / Tumors / Young Adult / Yttrium Radioisotopes - pharmacology / Yttrium Radioisotopes - therapeutic use