Journal Article

Meta-analysis of GWAS of over 16,000 individuals with autism spectrum disorder highlights a novel locus at 10q24.32 and a significant overlap with schizophrenia

Hansen, Christine Søholm, Anttila, Verneri, Schellenberg, Gerard D, Levitt, Pat, Reichert, Jennifer, Mors, Ole, Mane, Shrikant M, Minshew, Nancy, Waltes, Regina, Magalhaes, Tiago, Willsey, a Jeremy, Nurnberger, John I, Pejovic-Milovancevic, Milica, Iliadou, Bozenna, Zwaigenbaum, Lonnie, Lecouteur, Ann S, Svantesson, Oscar, Stefansson, Kari, Mattheisen, Manuel, Klei, Lambertus, Martin, Christa Lese, Vanengeland, Herman, The Psychiatric Genomics Consortium, The Autism Spectrum Disorders Working Group Of, Poultney, Christopher S, Mcgrew, Susan G, Leboyer, Marion, Yu, Timothy W, Sandin, Sven, Martsenkovsky, Igor, Thompson, Ann P, Szatmari, Peter, Anney, Richard J L, Pedersen, Marianne Giørtz, Martin, Donna M, Paterson, Andrew D, Hougaard, David M, Kolevzon, Alexander, Nordentoft, Merete, Rouleau, Guy A, Saemundsen, Evald, Pedersen, Carsten Bøcker, Werge, Thomas, Stefansson, Hreinn, Maestrini, Elena, Poustka, Fritz, Medland, Sarah E, Monaco, Anthony P, Regan, Regina, Moreno-De-Luca, Daniel, Klauck, Sabine M, Huang, Hailiang, Reichenberg, Abraham, Holmans, Peter, Wassink, Thomas H, Magnusson, Pall

2017

Overview

Background Over the past decade genome-wide association studies (GWAS) have been applied to aid in the understanding of the biology of traits. The success of this approach is governed by the underlying effect sizes carried by the true risk variants and the corresponding statistical power to observe such effects given the study design and sample size under investigation. Previous ASD GWAS have identified genome-wide significant (GWS) risk loci; however, these studies were of only of low statistical power to identify GWS loci at the lower effect sizes (odds ratio (OR) <1.15). Methods We conducted a large-scale coordinated international collaboration to combine independent genotyping data to improve the statistical power and aid in robust discovery of GWS loci. This study uses genome-wide genotyping data from a discovery sample (7387 ASD cases and 8567 controls) followed by meta-analysis of summary statistics from two replication sets (7783 ASD cases and 11359 controls; and 1369 ASD cases and 137308 controls). Results We observe a GWS locus at 10q24.32 that overlaps several genes including PITX3 , which encodes a transcription factor identified as playing a role in neuronal differentiation and CUEDC2 previously reported to be associated with social skills in an independent population cohort. We also observe overlap with regions previously implicated in schizophrenia which was further supported by a strong genetic correlation between these disorders (Rg = 0.23; P  = 9 × 10 −6 ). We further combined these Psychiatric Genomics Consortium (PGC) ASD GWAS data with the recent PGC schizophrenia GWAS to identify additional regions which may be important in a common neurodevelopmental phenotype and identified 12 novel GWS loci. These include loci previously implicated in ASD such as FOXP1 at 3p13, ATP2B2 at 3p25.3, and a ‘neurodevelopmental hub’ on chromosome 8p11.23. Conclusions This study is an important step in the ongoing endeavour to identify the loci which underpin the common variant signal in ASD. In addition to novel GWS loci, we have identified a significant genetic correlation with schizophrenia and association of ASD with several neurodevelopmental-related genes such as EXT1 , ASTN2 , MACROD2 , and HDAC4.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Adaptor Proteins, Signal Transducing / asperger-syndrome / Autism / Autism spectrum disorder / Autism Spectrum Disorder - diagnosis / Autism Spectrum Disorder - genetics / Autism Spectrum Disorder - physiopathology / Carrier Proteins - genetics / Case-Control Studies / Chromosomes, Human, Pair 10 - chemistry / copy number variation / de-novo mutations / Female / Forkhead Transcription Factors - genetics / Gene Expression / Gene-set analysis / genetic / Genetic aspects / Genetic correlation / Genetic Loci / Genetic Predisposition to Disease / Genetic susceptibility / Genetics / Genetics & Heredity / genome-wide association / Genome-Wide Association Study / Genomes / Heritability / Homeodomain Proteins - genetics / Human Genetics / Humans / intellectual disability / Life Sciences / Male / Medicine / Medicine & Public Health / Membrane Proteins - genetics / mental-retardation / Meta-analysis / Neurodevelopment / Neurologi / Neurology / neuronal migration / Neuropsychology / Neurosciences / Neurosciences & Neurology / Pediatrics / Pervasive developmental disorders / Plasma Membrane Calcium-Transporting ATPases - genetics / Psychiatry / Repressor Proteins - genetics / resource exchange / Risk factors / risk loci / Schizophrenia / Schizophrenia - diagnosis / Schizophrenia - genetics / Schizophrenia - physiopathology / Systematic review / Transcription Factors - genetics