Journal Article

Failure of Intravenous or Intracardiac Delivery of Mesenchymal Stromal Cells to Improve Outcomes after Focal Traumatic Brain Injury in the Female Rat

Lewis, Bobbi K., Turtzo, L. Christine, Dean, Dana D., Janes, Lindsay, Budde, Matthew D., Grunberg, Neil E., Gold, Eric M., Yarnell, Angela, Frank, Joseph A., Lescher, Jacob, Coppola, Tiziana

2015

Overview

Mesenchymal stromal cells secrete a variety of anti-inflammatory factors and may provide a regenerative medicine option for the treatment of traumatic brain injury. The present study investigates the efficacy of multiple intravenous or intracardiac administrations of rat mesenchymal stromal cells or human mesenchymal stromal cells in female rats after controlled cortical impact by in vivo MRI, neurobehavior, and histopathology evaluation. Neither intravenous nor intracardiac administration of mesenchymal stromal cells derived from either rats or humans improved MRI measures of lesion volume or neurobehavioral outcome compared to saline treatment. Few mesenchymal stromal cells (<0.0005% of injected dose) were found within 3 days of last dosage at the site of injury after either delivery route, with no mesenchymal stromal cells being detectable in brain at 30 or 56 days post-injury. These findings suggest that non-autologous mesenchymal stromal cells therapy via intravenous or intracardiac administration is not a promising treatment after focal contusion traumatic brain injury in this female rodent model.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Administration, Intravenous / Analgesics / Animals / Bone marrow / Brain / Brain - cytology / Brain - pathology / Brain injuries / Brain Injuries - therapy / Brain research / Cardiac Catheters / Care and treatment / Cell- and Tissue-Based Therapy - methods / Cells, Cultured / Clinical psychology / Cortex / Diagnosis / Disease Models, Animal / Drug dosages / Female / Gender / Head injuries / Health sciences / Histopathology / Humans / Image Processing, Computer-Assisted / Inflammation / Intravenous administration / Laboratory animals / Magnetic Resonance Imaging / Medical care / Medicine / Mesenchymal Stem Cell Transplantation - methods / Mesenchymal stem cells / Mesenchymal Stromal Cells - cytology / Mesenchyme / Neurosciences / Neurosurgery / Patient outcomes / Rats / Rats, Wistar / Regenerative medicine / Rodents / Stem cells / Stromal cells / Studies / Systematic review / Transplants & implants / Traumatic brain injury / Treatment Failure / United States