Journal Article

Anti-inflammatory and wound healing activities of calophyllolide isolated from Calophyllum inophyllum Linn

Nguyen, Binh Cao Quan, Huynh, Hieu Kim, Truong, Cong-Tri, Vo, Thanh-Niem Van, Nguyen, Van-Linh, Trinh, Dieu-Thuong Thi, Dao, Trong-Thuc, Bui, Chi-Bao, Nguyen, Van-Dan

2017

Overview

Due to the high-cost and limitations of current wound healing treatments, the search for alternative approaches or drugs, particularly from medicinal plants, is of key importance. In this study, we report anti-inflammatory and wound healing activities of the major calophyllolide (CP) compound isolated from Calophyllum inophyllum Linn. The results showed that CP had no effect on HaCaT cell viability over a range of concentrations. CP reduced fibrosis formation and effectively promoted wound closure in mouse model without causing body weight loss. The underlying molecular mechanisms of wound repair by CP was investigated. CP markedly reduced MPO activity, and increased M2 macrophage skewing, as shown by up-regulation of M2-related gene expression, which is beneficial to the wound healing process. CP treatment prevented a prolonged inflammatory process by down-regulation of the pro-inflammatory cytokines-IL-1β, IL-6, TNF-α, but up-regulation of the anti-inflammatory cytokine, IL-10. This study is the first to indicate a plausible role for CP in accelerating the process of wound healing through anti-inflammatory activity mechanisms, namely, by regulation of inflammatory cytokines, reduction in MPO, and switching of macrophages to an M2 phenotype. These findings may enable the utilization of CP as a potent therapeutic for cutaneous wound healing.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Animals / Anti-inflammatory agents / Anti-Inflammatory Agents - pharmacology / Biology and Life Sciences / Biomarkers - metabolism / Body weight / Body weight loss / Calophyllum - chemistry / Calophyllum inophyllum / Cell Survival - drug effects / Coumarins - isolation & purification / Coumarins - pharmacology / Cytokines / Cytokines - metabolism / Drugs / Fibroblasts / Fibrosis / Folk medicine / Gene expression / Gene Expression Regulation - drug effects / Growth factors / Health aspects / Humans / Inflammation / Inflammation Mediators - metabolism / Interleukin 10 / Interleukin 6 / Laboratories / Macrophages / Macrophages - drug effects / Macrophages - metabolism / Magnoliopsida / Male / Medicinal plants / Medicine / Medicine and Health Sciences / Methods / Mice / Molecular modelling / Peroxidase - metabolism / Pharmacy / Plant products / R&D / RAW 264.7 Cells / Research & development / Research and Analysis Methods / Rodents / Scars / Seeds - chemistry / Skin - drug effects / Skin - pathology / Sodium Dodecyl Sulfate / Spleen - drug effects / Spleen - pathology / Switching / Traditional medicine / Tumor necrosis factor-TNF / Tumor necrosis factor-α / Wound healing / Wound Healing - drug effects