Asset Details
Do you wish to reserve the book?
Successes and Short Comings in Four Years of an International External Quality Assurance Program for Animal Influenza Surveillance
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Successes and Short Comings in Four Years of an International External Quality Assurance Program for Animal Influenza Surveillance
Do you wish to request the book?
Successes and Short Comings in Four Years of an International External Quality Assurance Program for Animal Influenza Surveillance
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Successes and Short Comings in Four Years of an International External Quality Assurance Program for Animal Influenza Surveillance
2016
Overview
The US National institutes of Health-Centers of Excellence for Influenza Research and Surveillance is a research consortium that funds numerous labs worldwide to conduct influenza A surveillance in diverse animal species. There is no harmonization of testing procedures among these labs; therefore an external quality assurance (EQA) program was implemented to evaluate testing accuracy among labs in the program in 2012. Accurate detection of novel influenza A variants is crucial because of the broad host range and potentially high virulence of the virus in diverse species. Two molecular detection sample sets and 2 serology sample sets (one with avian origin isolates, and one with mammalian origin isolates each) were made available at approximately six month intervals. Participating labs tested the material in accordance with their own protocols. During a five year period a total of 41 labs from 23 countries ordered a total of 132 avian molecular, 121 mammalian molecular and 90 serology sample sets. Testing was completed by 111 individuals. Detection of type A influenza by RT-PCR was reliable with a pass rate (80% or greater agreement with expected results) of 86.6% for avian and 86.2% for mammalian origin isolates. However, identification of subtype by RT-PCR was relatively poor with 54.1% and 75.9% accuracy for avian and mammalian influenza isolates respectively. Serological testing had an overall pass rate of 86.9% and 22/23 labs used commercial ELISA kits. Based on the results of this EQA program six labs modified their procedures to improve accuracy and one lab identified an unknown equipment problem. These data represent the successful implementation of an international EQA program for an infectious disease; insights into the logistics and test design are also discussed.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Animals
/ Birds
/ Enzyme-linked immunosorbent assay
/ Enzymes
/ funding
/ Humans
/ Influenza in Birds - epidemiology
/ Influenza, Human - epidemiology
/ Mammals
/ Medicine and health sciences
/ Outcome Assessment (Health Care)
/ Poultry
/ Research and Analysis Methods
/ reverse transcriptase polymerase chain reaction
/ Serology
/ Virology
/ Viruses
