Journal Article

Chemical Composition and Biological Activity of Extracts Obtained by Supercritical Extraction and Ethanolic Extraction of Brown, Green and Red Propolis Derived from Different Geographic Regions in Brazil

Brandão, Hugo Neves, Silva, Danielle Figuerêdo da, Silva, Rejane Pina Dantas, Costa, Samantha Serra, Umsza-Guez, Marcelo Andres, Padilha, Francine Ferreira, Barreto, Gabriele de Abreu, Dellagostin, Odir Antônio, Henriques, João Antônio Pegas, Machado, Bruna Aparecida Souza, Rocha, José Luiz Carneiro da

2016

Overview

The variations in the chemical composition, and consequently, on the biological activity of the propolis, are associated with its type and geographic origin. Considering this fact, this study evaluated propolis extracts obtained by supercritical extraction (SCO2) and ethanolic extraction (EtOH), in eight samples of different types of propolis (red, green and brown), collected from different regions in Brazil. The content of phenolic compounds, flavonoids, in vitro antioxidant activity (DPPH and ABTS), Artepillin C, p-coumaric acid and antimicrobial activity against two bacteria were determined for all extracts. For the EtOH extracts, the anti-proliferative activity regarding the cell lines of B16F10, were also evaluated. Amongst the samples evaluated, the red propolis from the Brazilian Northeast (states of Sergipe and Alagoas) showed the higher biological potential, as well as the larger content of antioxidant compounds. The best results were shown for the extracts obtained through the conventional extraction method (EtOH). However, the highest concentrations of Artepillin C and p-coumaric acid were identified in the extracts from SCO2, indicating a higher selectivity for the extraction of these compounds. It was verified that the composition and biological activity of the Brazilian propolis vary significantly, depending on the type of sample and geographical area of collection.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Acids / Animals / Anti-Infective Agents - chemistry / Anti-Infective Agents - isolation & purification / Anti-Infective Agents - pharmacology / Antimicrobial activity / Antineoplastic Agents, Phytogenic - chemistry / Antineoplastic Agents, Phytogenic - isolation & purification / Antineoplastic Agents, Phytogenic - toxicity / Antioxidants / Antioxidants - chemistry / Antioxidants - isolation & purification / Antioxidants - pharmacology / Apoptosis / Bacteria / Biological activity / Brazil / Breast cancer / Cell Line, Tumor / Cell lines / Cell Proliferation - drug effects / Chemical composition / Chemical properties / Chromatography, High Pressure Liquid / Chromatography, Supercritical Fluid / Comparative analysis / Coumaric acid / Coumaric Acids - chemistry / Coumaric Acids - isolation & purification / Coumaric Acids - pharmacology / Escherichia coli - drug effects / Ethanol / Ethanol - chemistry / Extraction (Chemistry) / Flavonoids / Flavonoids - chemistry / Flavonoids - isolation & purification / Flavonoids - pharmacology / Food / Kinases / Mice / Microscopy, Electron, Scanning / p-Coumaric acid / Pharmacy / Phenolic compounds / Phenols / Phenols - chemistry / Phenols - isolation & purification / Phenols - pharmacology / Phenylpropionates - chemistry / Phenylpropionates - isolation & purification / Phenylpropionates - pharmacology / Physiological aspects / Propionates / Propolis / Propolis - analysis / Propolis - isolation & purification / Propolis - pharmacology / Prostate cancer / Staphylococcus aureus - drug effects