Journal Article

Calcium salts of long-chain fatty acids from linseed oil decrease methane production by altering the rumen microbiome in vitro

Oishi, Kazato, Aoki, Hirotatsu, Murayama, Masayuki, Yoshida, Takashi, Tominaga, Kento, Sato, Yoshiaki, Hirooka, Hiroyuki, Kumagai, Hajime

2020

Overview

Calcium salts of long-chain fatty acids (CSFA) from linseed oil have the potential to reduce methane (CH 4 ) production from ruminants; however, there is little information on the effect of supplementary CSFA on rumen microbiome as well as CH 4 production. The aim of the present study was to evaluate the effects of supplementary CSFA on ruminal fermentation, digestibility, CH 4 production, and rumen microbiome in vitro . We compared five treatments: three CSFA concentrations—0% (CON), 2.25% (FAL) and 4.50% (FAH) on a dry matter (DM) basis—15 mM of fumarate (FUM), and 20 mg/kg DM of monensin (MON). The results showed that the proportions of propionate in FAL, FAH, FUM, and MON were increased, compared with CON (P < 0.05). Although DM and neutral detergent fiber expressed exclusive of residual ash (NDFom) digestibility decreased in FAL and FAH compared to those in CON (P < 0.05), DM digestibility-adjusted CH 4 production in FAL and FAH was reduced by 38.2% and 63.0%, respectively, compared with that in CON (P < 0.05). The genera Ruminobacter , Succinivibri o, Succiniclasticum , Streptococcus , Selenomonas . 1 , and Megasphaera , which are related to propionate production, were increased (P < 0.05), while Methanobrevibacter and protozoa counts, which are associated with CH 4 production, were decreased in FAH, compared with CON (P < 0.05). The results suggested that the inclusion of CSFA significantly changed the rumen microbiome, leading to the acceleration of propionate production and the reduction of CH 4 production. In conclusion, although further in vivo study is needed to evaluate the reduction effect on rumen CH 4 production, CSFA may be a promising candidate for reduction of CH 4 emission from ruminants.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Animal Feed / Animal Nutritional Physiological Phenomena / Animals / Barley / Biology and Life Sciences / Body Weight / Breeding of animals / Calcium / Calcium - chemistry / Calcium compounds / Carbon dioxide / Cluster Analysis / Dairy cattle / Detergents / Digestibility / Digestion / DNA, Bacterial - metabolism / Dry matter / Emissions control / Fatty acids / Fatty Acids - chemistry / Fermentation / Fumarates - chemistry / Gases / In Vitro Techniques / In vivo methods and tests / Laboratories / Linseed oil / Linseed Oil - chemistry / Lipids / Livestock / Medicine and Health Sciences / Megasphaera - metabolism / Methane / Methane - chemistry / Methane production / Microbiomes / Microbiota / Microorganisms / Monensin / Monensin - chemistry / Oils & fats / Physical Sciences / Physiological aspects / Propionic acid / Protozoa / RNA, Ribosomal, 16S - metabolism / Rumen / Rumen - microbiology / Ruminants / Salts / Salts - chemistry / Selenomonas - metabolism / Sheep / Silage - analysis / Streptococcus - metabolism