Journal Article

Green-synthesized zinc oxide nanoparticles by Enterobacter sp.: unveiling characterization, antimicrobial potency, and alleviation of copper stress in Vicia faba (L.) plants

Afifi, Salsabil S., Abo-Shanab, Walaa A., Elsilk, Sobhy E., El-Shenody, Rania A.

2024

Overview

Background The biosynthesis of zinc oxide nanoparticles (ZnO NPs) using Enterobacter sp. and the evaluation of their antimicrobial and copper stress (Cu + 2 )-reducing capabilities in Vicia faba (L.) plants. The green-synthesized ZnO NPs were validated using X-ray powder diffraction (XRD); Fourier transformed infrared (FTIR), Ultraviolet-Visible spectroscopy (UV-Vis), Transmission electron microscope (TEM) and scanning electron microscopy (SEM) techniques. ZnO NPs could serve as an improved bactericidal agent for various biological applications. as well as these nanoparticles used in alleviating the hazardous effects of copper stress on the morphological and physiological traits of 21-day-old Vicia faba (L.) plants. Results The results revealed that different concentrations of ZnO NPs (250, 500, or 1000 mg L -1 ) significantly alleviated the toxic effects of copper stress (100 mM CuSO 4 ) and increased the growth parameters, photosynthetic efficiency (Fv/Fm), and pigments (Chlorophyll a and b) contents in Cu-stressed Vicia faba (L.) seedlings. Furthermore, applying high concentration of ZnO NPs (1000 mg L -1 ) was the best dose in maintaining the levels of antioxidant enzymes (CAT, SOD, and POX), total soluble carbohydrates, total soluble proteins, phenolic and flavonoid in all Cu-stressed Vicia faba (L.) seedlings. Additionally, contents of Malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ) were significantly suppressed in response to high concentrations of ZnO NPs (1000 mg L -1 ) in all Cu-stressed Vicia faba (L.) seedlings. Also, it demonstrates strong antibacterial action (0.9 mg/ml) against various pathogenic microorganisms. Conclusions The ZnO NPs produced in this study demonstrated the potential to enhance plant detoxification and tolerance mechanisms, enabling plants to better cope with environmental stress. Furthermore, these nanoparticles could serve as an improved bactericidal agent for various biological applications.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abiotic stress tolerance in plants / Agriculture / Anti-Bacterial Agents - pharmacology / antibacterial properties / Antiinfectives and antibacterials / Antimicrobial / Antimicrobial agents / Antioxidants / Antioxidants - metabolism / Bacteria / Bioflavonoids / Biomedical and Life Sciences / Biosynthesis / Carbohydrates / Chlorophyll / Copper / Copper stress / Detoxification / Diffraction / E coli / Electron microscopy / Enterobacter / Enterobacter - drug effects / Enterobacter - metabolism / Enterobacter sp / Enterobacteriaceae / Environmental stress / Enzymes / Flavones / Flavonoids / Green Chemistry Technology / Heavy metals / Hydrogen peroxide / Life Sciences / malondialdehyde / Metal Nanoparticles - chemistry / Methylene blue / Microorganisms / Nanomaterials / Nanoparticles / Nanoparticles - chemistry / Peroxides / Phenolic compounds / Phenols / Photocatalysis / photosynthesis / Physiological aspects / Physiological effects / Pigments / Plant Sciences / Probiotics / Properties / Scanning electron microscopy / Seedlings / Seedlings - drug effects / Spectrum analysis / Stress (Psychology) / Stress, Physiological - drug effects / Tetracycline / Tetracyclines / toxicity / Transmission electron microscopes / Tree Biology / ultraviolet-visible spectroscopy / Vicia faba / Vicia faba - drug effects / Vicia faba - metabolism / X ray powder diffraction / X-ray diffraction / X-rays / Zinc oxide / Zinc Oxide - pharmacology / Zinc oxides / ZnO NPs