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Overexpression of a novel small auxin-up RNA gene, OsSAUR11, enhances rice deep rootedness
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Overexpression of a novel small auxin-up RNA gene, OsSAUR11, enhances rice deep rootedness
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Overexpression of a novel small auxin-up RNA gene, OsSAUR11, enhances rice deep rootedness
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Journal Article
Overexpression of a novel small auxin-up RNA gene, OsSAUR11, enhances rice deep rootedness
2023
Overview
Background
Deep rooting is an important factor affecting rice drought resistance. However, few genes have been identified to control this trait in rice. Previously, we identified several candidate genes by QTL mapping of the ratio of deep rooting and gene expression analysis in rice.
Results
In the present work, we cloned one of these candidate genes,
OsSAUR11
, which encodes a small auxin-up RNA (SAUR) protein. Overexpression of
OsSAUR11
significantly enhanced the ratio of deep rooting of transgenic rice, but knockout of this gene did not significantly affect deep rooting. The expression of
OsSAUR11
in rice root was induced by auxin and drought, and OsSAUR11-GFP was localized both in the plasma membrane and cell nucleus. Through an electrophoretic mobility shift assay and gene expression analysis in transgenic rice, we found that the transcription factor OsbZIP62 can bind to the promoter of
OsSAUR11
and promote its expression. A luciferase complementary test showed that OsSAUR11 interacts with the protein phosphatase OsPP36. Additionally, expression of several auxin synthesis and transport genes (e.g.,
OsYUC5
and
OsPIN2
) were down-regulated in
OsSAUR11
-overexpressing rice plants.
Conclusions
This study revealed a novel gene
OsSAUR11
positively regulates deep rooting in rice, which provides an empirical basis for future improvement of rice root architecture and drought resistance.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Auxins
/ Biomedical and Life Sciences
/ Cloning
/ Drought
/ Genes
/ genetically modified organisms
/ Genomes
/ Growth
/ Mutation
/ Plants
/ Proteins
/ Rice
/ RNA
/ Rooting
