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Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods
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Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods
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Journal Article
Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods
2017
Overview
In this study, different molecular typing tools were applied to characterize 95 Salmonella enterica blood isolates collected between 2008 and 2013 from patients at nine public hospitals in Lima, Peru. Combined results of multiplex PCR serotyping, two- and seven-loci multilocus sequence typing (MLST) schemes, serotyping, IS200 amplification and RAPD fingerprints, showed that these infections were caused by eight different serovars: Enteritidis, Typhimurium, Typhi, Choleraesuis, Dublin, Paratyphi A, Paratyphi B and Infantis. Among these, Enteritidis, Typhimurium and Typhi were the most prevalent, representing 45, 36 and 11% of the isolates, respectively. Most isolates (74%) were not resistant to ten primarily used antimicrobial drugs; however, 37% of the strains showed intermediate susceptibility to ciprofloxacin (ISC). Antimicrobial resistance integrons were carried by one Dublin (dfra1 and aadA1) and two Infantis (aadA1) isolates. The two Infantis isolates were multidrug resistant and harbored a large megaplasmid. Amplification of spvC and spvRA regions showed that all Enteritidis (n = 42), Typhimurium (n = 34), Choleraesuis (n = 3) and Dublin (n = 1) isolates carried the Salmonella virulence plasmid (pSV). We conclude that the classic serotyping method can be substituted by the multiplex PCR and, when necessary, sequencing of only one or two loci of the MLST scheme is a valuable tool to confirm the results. The effectiveness and feasibility of different typing tools is discussed.
Publisher
Public Library of Science (PLoS),Public Library of Science
Subject
/ Analysis
/ Animals
/ Antigens
/ Antiinfectives and antibacterials
/ Drugs
/ Genes
/ Genomes
/ Genomics
/ https://purl.org/pe-repo/ocde/ford#1.06.01
/ Humans
/ Identification and classification
/ Medicine
/ Medicine and Health Sciences
/ Methods
/ Multiplex Polymerase Chain Reaction
/ Plasmids
/ Q
/ R
/ Research and Analysis Methods
/ Salmonella enterica - genetics
/ Salmonella enterica - isolation & purification
/ Salmonella enterica/genetics/isolation & purification
/ Science
