Journal Article

TaSnRK3.23B, a CBL-interacting protein kinase of wheat, confers drought stress tolerance by promoting ROS scavenging in Arabidopsis

Song, Jinghan, Liu, Yike, Zhang, Huadong, Zhu, Zhanwang, Chen, Sheng, Li, Yan, Liu, Yide, Dong, Feiyan, Li, Yaqian, Wang, Shuailei

2025

Overview

Background Sucrose non-fermenting-1-related protein kinases (SnRKs) have been implicated in plant growth and stress responses. Although SnRK3.23 is known to be involved in drought stress, the underlying mechanism of resistance differs between Arabidopsis and rice, and little is known about its function in wheat. Results In the current work, TaSnRK3.23B was detected on the cell membrane and in the nucleus. TaSnRK3.23B overexpression in Arabidopsis promoted reactive oxygen species (ROS) scavenging via the accumulation of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) and then conferred significant tolerance to drought. The prediction analysis, yeast two-hybrid, and bimolecular fluorescence complementation (BiFC) assays revealed that TaSnRK3.23B interacted with TaCBL2B and TaCBL6B, which are calcineurin B-like (CBL) proteins. The predicted model demonstrated that TaSnRK3 subfamily proteins participate in Ca 2+ signaling mediated by TaCBL2B/TaCBL6B and subsequently provide drought stress tolerance by promoting ROS scavenging in wheat. Conclusions Altogether, the obtained findings contribute to a better understanding of the functions of SnRK3.23 in wheat and offer genetic suggestions for improving drought resistance of wheat.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Abiotic stress / Abscisic acid / Agriculture / Analysis / Arabidopsis / Arabidopsis - genetics / Arabidopsis - metabolism / Arabidopsis - physiology / Biomedical and Life Sciences / Calcineurin / calcium / Calcium ions / Calcium signalling / Calcium-Binding Proteins - genetics / Calcium-Binding Proteins - metabolism / Catalase / Cbl protein / CBL proteins / Cell membranes / Cellular stress response / Cold / Complementation / Crop breeding for drought stress tolerance / Diseases and pests / Drought / Drought resistance / Drought stress / drought tolerance / Droughts / Ectopic expression / fluorescence / Gene Expression Regulation, Plant / Genes / Genetic aspects / Germination / Growth / Health aspects / Kinases / Life Sciences / Methods / Peroxidase / Phosphorylation / Phylogenetics / Plant growth / Plant Proteins - genetics / Plant Proteins - metabolism / Plant Sciences / Plants, Genetically Modified / prediction / Protein kinase / Protein kinases / Protein Serine-Threonine Kinases - genetics / Protein Serine-Threonine Kinases - metabolism / Proteins / Reactive oxygen species / Reactive Oxygen Species - metabolism / rice / Salt / Salt stress (Botany) / Scavenging / Stress, Physiological / subfamily / Sucrose / Superoxide dismutase / TaSnRK3.23B / Tobacco / Tree Biology / Triticum - enzymology / Triticum - genetics / Triticum - metabolism / Triticum - physiology / two hybrid system techniques / water stress / Wheat / Yeast