Journal Article

Oral Administration of P. gingivalis Induces Dysbiosis of Gut Microbiota and Impaired Barrier Function Leading to Dissemination of Enterobacteria to the Liver

Minagawa, Takayoshi, Takahashi, Naoki, Kato, Tamotsu, Arimatsu, Kei, Matsuda, Yumi, Nakajima, Mayuka, Yamazaki, Kazuhisa, Ohno, Hiroshi

2015

Overview

Although periodontitis has been implicated as a risk factor for various systemic diseases, the precise mechanisms by which periodontitis induces systemic disease remain to be elucidated. We have previously revealed that repeated oral administration of Porphyromonas gingivalis elicits endotoxemia via changes in the gut microbiota of the ileum, and thereby induces systemic inflammation and insulin resistance. However, it is not clear to what extent a single administration of P. gingivalis could affect gut microbiota composition, gut barrier function, and subsequent influx of gut microbiota into the liver. Therefore, in the present study, C57BL/6 mice were orally administered P. gingivalis (strain W83) once and compared to sham-inoculated mice. The phylogenetic structure and diversity of microbial communities in the gut and liver were analyzed by pyrosequencing the 16S ribosomal RNA genes. Serum endotoxin activity was determined by a Limulus amebocyte lysate test. Gene expression in the intestine and expression of 16S rRNA genes in the blood and liver were examined by quantitative polymerase chain reaction. Administration of P. gingivalis significantly altered gut microbiota, with an increased proportion of phylum Bacteroidetes, a decreased proportion of phylum Firmicutes, and increased serum endotoxin levels. In the intestinal tissues, gene expression of tjp-1 and occludin, which are involved in intestinal permeability, were downregulated. Higher amounts of bacterial DNA were detected in the liver of infected mice. Importantly, changes in gut microbiota preceded systemic inflammatory changes. These results further support the idea that disturbance of the gut microbiota composition by orally derived periodontopathic bacteria may be a causal mechanism linking periodontitis and systemic disease.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Administration, Oral / Animal tissues / Animals / Bacteria / Bacteroidaceae Infections - complications / Bacteroidaceae Infections - microbiology / Deoxyribonucleic acid / Diabetes / Digestive system / Digestive tract / Disease / DNA / Dysbacteriosis / Dysbiosis - etiology / Dysbiosis - metabolism / Dysbiosis - pathology / Ecosystems / Endotoxemia / Endotoxemia - etiology / Endotoxemia - metabolism / Endotoxemia - pathology / Endotoxins / Enterobacteriaceae - physiology / Enterobacteriaceae Infections - etiology / Enterobacteriaceae Infections - metabolism / Enterobacteriaceae Infections - pathology / Feces - microbiology / Gastrointestinal Microbiome / Gastrointestinal tract / Gene expression / Genes / Gum disease / Health promotion / Health risks / Humans / Ileum / Immune system / Immunology / Inflammation / Inflammation - etiology / Inflammation - metabolism / Inflammation - pathology / Inflammation Mediators - metabolism / Insulin / Intestinal microflora / Intestine / Laboratory animals / Liver / Liver - microbiology / Male / Metabolism / Metabolites / Mice / Mice, Inbred C57BL / Microbial activity / Microbiota / Microbiota (Symbiotic organisms) / Microorganisms / Oral administration / Periodontitis / Permeability / Phylogeny / Physiology / Polymerase chain reaction / Porphyromonas gingivalis - physiology / Ribonucleic acid / Risk factors / RNA / RNA, Ribosomal, 16S / rRNA 16S / Science / University graduates