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Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential
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Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential
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Journal Article
Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential
2011
Overview
Background
To expand on the range of products which can be obtained from lignocellulosic biomass, the lignin component should be utilized as feedstock for value-added chemicals such as substituted aromatics, instead of being incinerated for heat and energy. Enzymes could provide an effective means for lignin depolymerization into products of interest. In this study, soil bacteria were isolated by enrichment on Kraft lignin and evaluated for their ligninolytic potential as a source of novel enzymes for waste lignin valorization.
Results
Based on 16S rRNA gene sequencing and phenotypic characterization, the organisms were identified as
Pandoraea norimbergensis
LD001,
Pseudomonas
sp LD002 and
Bacillus
sp LD003. The ligninolytic capability of each of these isolates was assessed by growth on high-molecular weight and low-molecular weight lignin fractions, utilization of lignin-associated aromatic monomers and degradation of ligninolytic indicator dyes.
Pandoraea norimbergensis
LD001 and
Pseudomonas
sp. LD002 exhibited best growth on lignin fractions, but limited dye-decolourizing capacity.
Bacillus
sp. LD003, however, showed least efficient growth on lignin fractions but extensive dye-decolourizing capacity, with a particular preference for the recalcitrant phenothiazine dye class (Azure B, Methylene Blue and Toluidene Blue O).
Conclusions
Bacillus
sp. LD003 was selected as a promising source of novel types of ligninolytic enzymes. Our observations suggested that lignin mineralization and depolymerization are separate events which place additional challenges on the screening of ligninolytic microorganisms for specific ligninolytic enzymes.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Bacillus
/ Bacillus - isolation & purification
/ Bacteria
/ Biodegradation, Environmental
/ Biomass
/ Biomedical Engineering/Biotechnology
/ Burkholderiaceae - enzymology
/ Burkholderiaceae - isolation & purification
/ Chemistry and Materials Science
/ Color
/ Coloring Agents - metabolism
/ Dyes
/ Enzymes
/ Plant Breeding/Biotechnology
/ Polycyclic aromatic hydrocarbons
/ Pseudomonas - isolation & purification
/ RNA, Ribosomal, 16S - genetics
