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Periodic detection and disinfection maintenance of dental unit waterlines in dental simulation head model laboratories
by
Wang, Zimo
, Li, Biyao
, Hu, Yuya
, Wang, Yiqi
, Zhang, Chengqi
, Peng, Xujie
, Feng, Jianying
in
631/326
/ 692/1537
/ 692/700
/ Bacteria - drug effects
/ Bacteria - isolation & purification
/ Chlorine
/ Chlorine Compounds - pharmacology
/ Chlorine dioxide
/ Colonies
/ Colony Count, Microbial
/ Contamination
/ Dental Equipment - microbiology
/ Dental simulation head model laboratory
/ Dental unit waterlines
/ Disinfectants
/ Disinfectants - pharmacology
/ Disinfection
/ Disinfection & disinfectants
/ Disinfection - methods
/ Equipment Contamination - prevention & control
/ High-throughput sequencing
/ Humanities and Social Sciences
/ Humans
/ Laboratories
/ Microbial contamination
/ Models, Dental
/ multidisciplinary
/ Organic compounds
/ Organochlorine compounds
/ Organochlorine disinfectant
/ Oxides
/ Science
/ Science (multidisciplinary)
/ Simulation
/ Syringes
/ Water analysis
/ Water Microbiology
/ Water pollution
/ Water sampling
2025
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Periodic detection and disinfection maintenance of dental unit waterlines in dental simulation head model laboratories
by
Wang, Zimo
, Li, Biyao
, Hu, Yuya
, Wang, Yiqi
, Zhang, Chengqi
, Peng, Xujie
, Feng, Jianying
in
631/326
/ 692/1537
/ 692/700
/ Bacteria - drug effects
/ Bacteria - isolation & purification
/ Chlorine
/ Chlorine Compounds - pharmacology
/ Chlorine dioxide
/ Colonies
/ Colony Count, Microbial
/ Contamination
/ Dental Equipment - microbiology
/ Dental simulation head model laboratory
/ Dental unit waterlines
/ Disinfectants
/ Disinfectants - pharmacology
/ Disinfection
/ Disinfection & disinfectants
/ Disinfection - methods
/ Equipment Contamination - prevention & control
/ High-throughput sequencing
/ Humanities and Social Sciences
/ Humans
/ Laboratories
/ Microbial contamination
/ Models, Dental
/ multidisciplinary
/ Organic compounds
/ Organochlorine compounds
/ Organochlorine disinfectant
/ Oxides
/ Science
/ Science (multidisciplinary)
/ Simulation
/ Syringes
/ Water analysis
/ Water Microbiology
/ Water pollution
/ Water sampling
2025
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Periodic detection and disinfection maintenance of dental unit waterlines in dental simulation head model laboratories
by
Wang, Zimo
, Li, Biyao
, Hu, Yuya
, Wang, Yiqi
, Zhang, Chengqi
, Peng, Xujie
, Feng, Jianying
in
631/326
/ 692/1537
/ 692/700
/ Bacteria - drug effects
/ Bacteria - isolation & purification
/ Chlorine
/ Chlorine Compounds - pharmacology
/ Chlorine dioxide
/ Colonies
/ Colony Count, Microbial
/ Contamination
/ Dental Equipment - microbiology
/ Dental simulation head model laboratory
/ Dental unit waterlines
/ Disinfectants
/ Disinfectants - pharmacology
/ Disinfection
/ Disinfection & disinfectants
/ Disinfection - methods
/ Equipment Contamination - prevention & control
/ High-throughput sequencing
/ Humanities and Social Sciences
/ Humans
/ Laboratories
/ Microbial contamination
/ Models, Dental
/ multidisciplinary
/ Organic compounds
/ Organochlorine compounds
/ Organochlorine disinfectant
/ Oxides
/ Science
/ Science (multidisciplinary)
/ Simulation
/ Syringes
/ Water analysis
/ Water Microbiology
/ Water pollution
/ Water sampling
2025
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Periodic detection and disinfection maintenance of dental unit waterlines in dental simulation head model laboratories
Journal Article
Periodic detection and disinfection maintenance of dental unit waterlines in dental simulation head model laboratories
2025
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Overview
Dental simulation head model laboratories are crucial for clinical simulation training for stomatological students, yet the maintenance of their dental unit waterlines (DUWLs) has been overlooked. This study investigated water contamination in DUWLs within these laboratories and proposed solutions. Water samples were collected from 12 dental chairs in three laboratories at three time points: the beginning, middle, and end of the semester. At the start of the semester, severe contamination was observed, with colony counts of 11,586
1715 CFU/ml for high-speed handpieces and 5375
874 CFU/ml for three ways syringes. As the semester progressed, colony counts gradually decreased but remained above clinical thresholds. Both 20 mg/L organochlorine disinfectant and 20 mg/L chlorine dioxide were effective in reducing bacterial contamination below standard ranges three days post-disinfection. Microbial diversity analysis revealed
Proteobacteria
and
Bacteroidota
as the dominant bacterial phyla, with
Ascomycota
as the dominant fungal phylum. Potentially pathogenic bacteria such as
Pseudomonas
,
Burkholderia-Caballeronia-Paraburkholderia
,
Ralstonia
,
Mycobacterium
,
Legionella
,
Paenibacillus
,
Streptomyces
,
Acinetobacter
, and
Prevotella
, as well as fungi like
Fusarium
and
Penicillium
, were detected. Therefore, urgent attention is needed to address DUWL contamination in dental laboratories, and it is recommended to disinfect DUWLs using either 20 mg/L organochlorine disinfectant or 20 mg/L chlorine dioxide every three days.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 692/1537
/ 692/700
/ Bacteria - isolation & purification
/ Chlorine
/ Chlorine Compounds - pharmacology
/ Colonies
/ Dental Equipment - microbiology
/ Dental simulation head model laboratory
/ Disinfectants - pharmacology
/ Disinfection & disinfectants
/ Equipment Contamination - prevention & control
/ Humanities and Social Sciences
/ Humans
/ Oxides
/ Science
/ Syringes
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