Journal Article

The association of lipid metabolism relative gene polymorphisms and ischemic stroke in Han and Uighur population of Xinjiang

Dila, Na-mu, Liu, Ling-yun, Mao, Jie-ping, Hu, Liang, Li, You-mei, Yue, Yun-hua, Yang, Xiao-ying

2017

Overview

Background The present study is aimed to evaluate difference of lipid metabolism related gene single nucleotide polymorphisms (SNPs) with ischemic stroke (IS) in Han and Uighur population of Xinjiang, China. Methods Four hundred eight patients with ischemic stroke and 347 unrelated healthy individuals of age and sex matched were genotyped for Apolipoprotein A5 (ApoA5), lipoprotein lipase (LPL), Cholesteryl ester transfer protein (CETP) and low-density lipoprotein receptor (LDL-R) genes. Their mutation difference was analyzed by SNaP shot techniques. GeneMapper4.1 SPSS20.0 software was used for data management and analysis. Using a single locus analysis, the distribution difference of genotype loci in ischemic stroke cases and controls were detected to assess the genetic risk factors of ischemic stroke. Results Significance differences of genotype distribution in ischemic stroke cases and controls were observed in LDLR rs688 in Han and Uighur population in recessive model from analysis of single gene locus. It also was found that dramatic difference of triglyceride (TG) of LPL rs328 and systolic blood pressure in CETP rs708277 of total population. In binary logistic regression analysis of total studied population, ischemic stroke was observed significantly associated with LDLR rs688 both addictive model (TT/CC, adjusted OR = 1.47, 95% CI = 1.04–2.07) and recessive model (TT/CT + CC, adjusted Odds ratio (OR) = 2.66, 95% Confidence Interval (CI) = 1.37–5.14). In Han population, ischemic stroke was observed significantly associated with rs688 both in addictive model (TT/CC, adjusted OR = 3.27, 95% CI = 1.06–10.05). In Uighur population, no significant association was found between gene polymorphisms and the risk of ischemic stroke. Combined analysis of multiple gene and loci, interaction effects of LDLR rs688 C/T, ApoA5 rs662799 A/G and CETP rs708272 C/T denoted a significant influence on IS susceptibility. Conclusion Single nucleotide polymorphisms of lipid metabolism relative gene were significantly associated with the morbidity of ischemic stroke in Han population. The interaction effects of rs688 C/T with ApoA5 rs662799 A/G and CETP rs708272 C/T promoted the occurrence of IS.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Aged / Alleles / Apolipoprotein / Apolipoprotein A-V - genetics / Apolipoprotein A-V - metabolism / Apolipoproteins / Asian Continental Ancestry Group / Biomedical and Life Sciences / Blood pressure / Brain Ischemia - genetics / Brain Ischemia - metabolism / Cardiovascular disease / China / Cholesterol / Cholesterol Ester Transfer Proteins - genetics / Cholesterol Ester Transfer Proteins - metabolism / Cholesteryl ester transfer protein / Clinical Nutrition / Data processing / Deoxyribonucleic acid / Diabetes / DNA / Ethnicity / Female / Genes / Genetic aspects / Genetic polymorphisms / Genetic Predisposition to Disease - genetics / Genetic testing / Genotype / Han (Chinese people) / Health aspects / Humans / Hypertension / Ischemia / Ischemic stroke / Life Sciences / Lipase / Lipid metabolism / Lipid Metabolism - genetics / Lipid Metabolism - physiology / Lipidology / Lipids / Lipoprotein lipase / Lipoprotein Lipase - genetics / Lipoprotein Lipase - metabolism / Low density lipoprotein / Low density lipoprotein receptor / Low density lipoprotein receptors / Male / Medical Biochemistry / Metabolism / Middle Aged / Morbidity / Mutation / Polymorphism, Single Nucleotide - genetics / Population / Population studies / Receptor density / Risk factors / Single nucleotide polymorphism / Statistical analysis / Stroke / Stroke - genetics / Stroke - metabolism / Uighurs