Asset Details
Do you wish to reserve the book?
Progenitor identification and SARS-CoV-2 infection in human distal lung organoids
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Progenitor identification and SARS-CoV-2 infection in human distal lung organoids
Do you wish to request the book?
Progenitor identification and SARS-CoV-2 infection in human distal lung organoids
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Progenitor identification and SARS-CoV-2 infection in human distal lung organoids
2020
Overview
The distal lung contains terminal bronchioles and alveoli that facilitate gas exchange. Three-dimensional in vitro human distal lung culture systems would strongly facilitate the investigation of pathologies such as interstitial lung disease, cancer and coronavirus disease 2019 (COVID-19) pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here we describe the development of a long-term feeder-free, chemically defined culture system for distal lung progenitors as organoids derived from single adult human alveolar epithelial type II (AT2) or KRT5
+
basal cells. AT2 organoids were able to differentiate into AT1 cells, and basal cell organoids developed lumens lined with differentiated club and ciliated cells. Single-cell analysis of
KRT5
+
cells in basal organoids revealed a distinct population of
ITGA6
+
ITGB4
+
mitotic cells, whose offspring further segregated into a
TNFRSF12A
hi
subfraction that comprised about ten per cent of KRT5
+
basal cells. This subpopulation formed clusters within terminal bronchioles and exhibited enriched clonogenic organoid growth activity. We created distal lung organoids with apical-out polarity to present ACE2 on the exposed external surface, facilitating infection of AT2 and basal cultures with SARS-CoV-2 and identifying club cells as a target population. This long-term, feeder-free culture of human distal lung organoids, coupled with single-cell analysis, identifies functional heterogeneity among basal cells and establishes a facile in vitro organoid model of human distal lung infections, including COVID-19-associated pneumonia.
A long-term culture method for organoids derived from single adult human lung cells is used to identify progenitor cells and study SARS-CoV-2 infection.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 13/106
/ 14
/ 14/1
/ 14/19
/ 14/32
/ 38
/ 38/91
/ 45
/ 82
/ ACE2
/ Alveolar Epithelial Cells - cytology
/ Alveolar Epithelial Cells - metabolism
/ Alveolar Epithelial Cells - virology
/ Alveoli
/ Angiotensin-converting enzyme 2
/ COVID-19
/ Culture
/ Genes
/ Humanities and Social Sciences
/ Humans
/ Influenza A Virus, H1N1 Subtype - growth & development
/ Influenza A Virus, H1N1 Subtype - physiology
/ Lumens
/ Lungs
/ Methods
/ Pneumonia, Viral - metabolism
/ Pneumonia, Viral - pathology
/ Polarity
/ SARS-CoV-2 - growth & development
/ Science
