Asset Details
Do you wish to reserve the book?
Influence of estradiol on bovine trophectoderm and uterine gene transcripts around maternal recognition of pregnancy
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Influence of estradiol on bovine trophectoderm and uterine gene transcripts around maternal recognition of pregnancy
Do you wish to request the book?
Influence of estradiol on bovine trophectoderm and uterine gene transcripts around maternal recognition of pregnancy
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Influence of estradiol on bovine trophectoderm and uterine gene transcripts around maternal recognition of pregnancy
2021
Overview
Embryo survival and pregnancy success is increased among animals that exhibit estrus prior to fixed time-artificial insemination, but there are no differences in conceptus survival to d16. The objective of this study was to determine effects of preovulatory estradiol on uterine transcriptomes, select trophectoderm (TE) transcripts, and uterine luminal fluid proteins. Beef cows/heifers were synchronized, artificially inseminated (d0), and grouped into either high (highE2) or low (lowE2) preovulatory estradiol. Uteri were flushed (d16); conceptuses and endometrial biopsies (n = 29) were collected. RNA sequencing was performed on endometrium. Real-time polymerase chain reaction (RT-PCR) was performed on TE (n = 21) RNA to measure relative abundance of IFNT, PTGS2, TM4SF1, C3, FGFR2, and GAPDH. Uterine fluid was analyzed using 2D Liquid Chromatography with tandem mass spectrometry-based Isobaric tags for relative and absolute quantitation (iTRAQ) method. RT-PCR data were analyzed using the MIXED procedure in SAS. There were no differences in messenger RNA (mRNA) abundances in TE, but there were 432 differentially expressed genes (253 downregulated, 179 upregulated) in highE2/conceptus versus lowE2/conceptus groups. There were also 48 differentially expressed proteins (19 upregulated, 29 downregulated); 6 of these were differentially expressed (FDR < 0.10) at the mRNA level. Similar pathways for mRNA and proteins included: calcium signaling, protein kinase A signaling, and corticotropin-releasing hormone signaling. These differences in uterine function may be preparing the conceptus for improved likelihood of survival after d16 among highE2 animals. Summary sentence Preovulatory estradiol did not impact conceptus survival to d16; however, it did influence uterine gene/protein expressions related to adhesion, endometrial remodeling, metabolism, and immune regulation, which may explain improved pregnancy success.
Publisher
Society for the Study of Reproduction,Oxford University Press
Subject
/ ACTH
/ Analysis
/ Animals
/ beef
/ calcium
/ cAMP-dependent protein kinase
/ Corticotropin releasing hormone
/ Embryo, Mammalian - embryology
/ Embryo, Mammalian - metabolism
/ Estrus
/ Female
/ Fibroblast growth factor receptor 2
/ Genes
/ Glyceraldehyde-3-phosphate dehydrogenase
/ Kinases
/ maternal recognition of pregnancy
/ Phenols
/ Proteins
/ quantitative polymerase chain reaction
/ Survival
/ Uterus
We currently cannot retrieve any items related to this title. Kindly check back at a later time.