Journal Article

Effects of Ectomycorrhizae and Hyphae on Soil Fungal Community Characteristics Across Forest Gap Positions

Liu, Sining, Li, Han, Tan, Bo, Xu, Lin, Zhang, Li, Wang, Lixia, Xu, Hongwei, Xu, Zhenfeng, Shen, Ya, You, Chengming

2024

Overview

The interactive effects of environmental heterogeneity caused by forest gaps and ectomycorrhizae on fungal community characteristics remain insufficiently explored. To address this knowledge gap, we established a three-year field manipulation experiment in a Picea asperata (Picea asperata Mast.) plantation located in the subalpine region of western Sichuan, China. Growth bags with three mesh sizes—1000 μm (allowing ectomycorrhizae and hyphae), 48 μm (excluding ectomycorrhizae), and 1 μm (excluding both)—were placed across forest gaps (closed canopy, gap edge, and gap center) to investigate how gap disturbances influence soil fungal communities via changes in ectomycorrhizal and hyphal turnover alongside soil physicochemical properties. Soil fungal α-diversity was significantly lower under closed-canopy conditions than at forest gap centers and remained unaffected by ectomycorrhizal and hyphal treatments. Particularly, species diversity increased by 9%, and phylogenetic diversity increased by 10% in forest gap centers compared to the closed canopy. In contrast, soil fungal β-diversity responded to both ectomycorrhizal/hyphal treatments (R2 = 0.061; p = 0.001) and forest gap positions (R2 = 0.033; p = 0.003). Pairwise comparative analyses revealed significant distinctions between treatments, concurrently excluding ectomycorrhizal and hyphal treatments versus other experimental treatments, as well as between closed-canopy conditions and forest gap centers. The fungal community was dominated by four major phyla: Ascomycota (25.6%–71.0%), Basidiomycota (17.7%–43.7%), Mortierellomycota (1.4%–24.5%), and Rozellomycota (0.4%–2.9%), the relative abundances of which were unaffected by either ectomycorrhizal/hyphal treatments or forest gap positions. The biomass of ectomycorrhizal and saprotrophic fungi showed no significant response to ectomycorrhizal/hyphal treatments. Notably, the exclusion of ectomycorrhizae and hyphae enhanced the significant correlations between fungal community characteristics and soil physicochemical properties. Hierarchical partitioning analysis revealed that the soil water content (SWC) and dissolved organic carbon content were the key determinants of soil fungal community characteristics beneath closed-canopy conditions. In contrast, at forest gap edges and centers, the fungal communities were predominantly shaped by the SWC and dissolved carbon and nitrogen contents. This study highlights the impacts of forest gap disturbances and ectomycorrhizal treatments on soil fungal communities, offering valuable insights for the sustainable management and biodiversity conservation of subalpine forest ecosystems.

• Publisher: MDPI AG
• Subject: Analysis / Biodiversity / Biodiversity conservation / Biological diversity / Biological diversity conservation / Canopies / Canopy gaps / Carbon / Carbon content / Comparative analysis / Decomposition / Dissolved organic carbon / Disturbances / Ecosystem management / Ecosystems / Ectomycorrhizas / Environmental effects / Forest ecosystems / Forest management / Forests / Fungi / Heterogeneity / Humidity / Hyphae / Hypotheses / Moisture content / Mountain ecology / Nitrogen / Phylogeny / Physicochemical properties / Picea asperata / Soil analysis / Soil conservation / Soil investigations / Soil microorganisms / Soil moisture / Soil properties / Soil water / Species diversity / Subalpine environments / Sustainability management / Terrestrial ecosystems / Water content