Asset Details
Do you wish to reserve the book?
Visualisation of in vivo protein synthesis during mycobacterial infection through 68GaGa-DOTA-puromycin µPET/MRI
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Visualisation of in vivo protein synthesis during mycobacterial infection through 68GaGa-DOTA-puromycin µPET/MRI
Do you wish to request the book?
Visualisation of in vivo protein synthesis during mycobacterial infection through 68GaGa-DOTA-puromycin µPET/MRI
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Visualisation of in vivo protein synthesis during mycobacterial infection through 68GaGa-DOTA-puromycin µPET/MRI
2024
Overview
Radiolabelled puromycin analogues will allow the quantification of protein synthesis through nuclear medicine-based imaging. A particularly useful application could be the non-invasive longitudinal visualisation of mycobacterial activity through direct quantification of puromycin binding. This study assesses the value of [
68
Ga]Ga-DOTA-puromycin in the visualisation of mycobacteria through positron emission tomography combined with magnetic resonance imaging (µPET/MRI). The radiopharmaceutical was produced by previously published and validated methods. [
68
Ga]Ga-DOTA-Puromycin imaging was performed on severe immunodeficient mice infected with Bacille Calmette-Guérin-derived
M. Bovis
(BCG). Acute and chronic infection stages were examined by µPET/MRI. A follow-up group of animals acted as controls (animals bearing
S
.
aureus
-derived infection and sterile inflammation) to assess tracer selectivity. [
68
Ga]Ga-DOTA-puromycin-µPET/MRI images revealed the acute, widespread infection within the right upper shoulder and armpit. Also, [
68
Ga]Ga-DOTA-puromycin signal sensitivity measured after a 12-week period was lower than that of [
18
F]FDG-PET in the same animals. A suitable correlation between normalised uptake values (NUV) and gold standard histopathological analysis confirms accurate tracer accumulation in viable bacteria. The radiopharmaceutical showed infection selectivity over inflammation but accumulated in both
M. Bovis
and
S. Aureus
, lacking pathogen specificity. Overall, [
68
Ga]Ga-DOTA-puromycin exhibits potential as a tool for non-invasive protein synthesis visualization, albeit without pathogen selectivity.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 692/4017
/ Animals
/ Female
/ Heterocyclic Compounds, 1-Ring - chemistry
/ Humanities and Social Sciences
/ Magnetic Resonance Imaging - methods
/ Mice
/ Mycobacterium Infections - diagnostic imaging
/ Mycobacterium Infections - microbiology
/ Positron emission tomography
/ Positron emission tomography/magnetic resonance imaging
/ Positron-Emission Tomography - methods
/ Proteins
/ Radiopharmaceuticals - chemistry
/ Science
