Asset Details
Do you wish to reserve the book?
Reprogramming of the histone H3.3 landscape in the early mouse embryo
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Reprogramming of the histone H3.3 landscape in the early mouse embryo
Do you wish to request the book?
Reprogramming of the histone H3.3 landscape in the early mouse embryo
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Reprogramming of the histone H3.3 landscape in the early mouse embryo
2021
Overview
Epigenetic reprogramming of the zygote involves dynamic incorporation of histone variant H3.3. However, the genome-wide distribution and dynamics of H3.3 during early development remain unknown. Here, we delineate the H3.3 landscapes in mouse oocytes and early embryos. We unexpectedly identify a non-canonical H3.3 pattern in mature oocytes and zygotes, in which local enrichment of H3.3 at active chromatin is suppressed and H3.3 is relatively evenly distributed across the genome. Interestingly, although the non-canonical H3.3 pattern forms gradually during oogenesis, it quickly switches to a canonical pattern at the two-cell stage in a transcription-independent and replication-dependent manner. We find that incorporation of H3.1/H3.2 mediated by chromatin assembly factor CAF-1 is a key process for the de novo establishment of the canonical pattern. Our data suggest that the presence of the non-canonical pattern and its timely transition toward a canonical pattern support the developmental program of early embryos.
Ultra-low-input native ChIP-seq analyses of genome-wide histone H3.3 distribution during early mouse development reveal a CAF-1-dependent transition from a non-canonical pattern in the oocyte and zygote to a canonical distribution at the two-cell stage.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ 64/60
/ 96/100
/ Animals
/ Biomedical and Life Sciences
/ Chromatin Assembly and Disassembly
/ Embryo, Mammalian - cytology
/ Embryonic Development - genetics
/ Embryos
/ Epigenesis, Genetic - genetics
/ Exoribonucleases - metabolism
/ Female
/ Gene Expression Regulation, Developmental
/ Genomes
/ Histones
/ Male
/ Mice
/ Oocytes
/ Repressor Proteins - metabolism
/ Switches
/ Zygote - growth & development
/ Zygotes
